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ab52032 has been referenced in 1 publications.
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All lanes : Anti-EBAG9 antibody (ab52032) at 0.2 µg/ml
Lane 1 : A431 lysate
Lane 2 : A431 lysate with Immunising peptide
Lysates/proteins at 35 µg per lane.
Predicted band size : 24 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
The band at 75kDa was successfully blocked by incubation with the immunising peptide.
Primary incubation was 1 hour. Detected by chemiluminescence.
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