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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-EBAG9 antibody
See all EBAG9 products (7) ...
Description
Goat polyclonal to EBAG9
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Dog, Xenopus laevis
Immunogen
Synthetic peptide: C-KEAQRLMKKEQN, corresponding to C terminal amino acids 195-206 of Human EBAG9
C-KEAQRLMK KEQN
Positive control
A431 lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
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Western blot - EBAG9 antibody (ab52032)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa). A minor band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
EBAG9 is a type III transmembrane protein that is predominantly located in the Golgi. It induces apoptosis of natural killer (NK)/T cells and plays a role in immune invasion, possibly in relation to Epstein-Bar virus infection. EBAG9 is abundantly expressed in prostate cancer cells when compared to normal epithelial cells, and immunodetection of EBAG9 expression can be a negative prognostic indicator for patients with prostate cancer.
Cellular localization
Golgi apparatus; Golgi apparatus membrane; single pass type III membrane protein.
Alternative names
- BAG9 antibody
- Cancer associated surface antigen antibody
- Cancer associated surface antigen RCAS1 antibody
see all
Database links
- Entrez Gene: 403500 Dog
- Entrez Gene: 9166 Human
- Entrez Gene: 55960 Mouse
- Entrez Gene: 299864 Rat
- Entrez Gene: 444828 Xenopus laevis
- Omim: 605772 Human
- SwissProt: Q865S0 Dog
- SwissProt: O00559 Human
see all
Anti-EBAG9 antibody images:
Western blot - EBAG9 antibody (ab52032)
All lanes : Anti-EBAG9 antibody (ab52032) at 0.2 µg/ml
Lane 1 : A431 lysate
Lane 2 : A431 lysate with Immunising peptide
Lysates/proteins at 35 µg per lane.
Predicted band size : 24 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
The band at 75kDa was successfully blocked by incubation with the immunising peptide.
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-EBAG9 antibody (ab52032)
This product has been referenced in:
- Tskitishvili Eet al. The effect of tumor-associated protein RCAS1 gene silencing on blood pressure and urinary protein excretion in pregnant mouse: a pilot study. Am J Obstet Gynecol 203:364.e6-364.e12 (2010). WB; Mouse.Read more (PubMed: 20579953) »
See 1 publication for this product
Publishing research using ab52032? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"