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Anti-EBF1 antibody (ab75249)

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Overview

Product name

Anti-EBF1 antibody
See all EBF1 products (4) ...

Description

Rabbit polyclonal to EBF1

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow, Dog, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Zebrafish

Immunogen

A synthetic peptide corresponding to a region of Human EBF1

Positive control

Fetal brain lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors

  • Western blot - EBF1 antibody (ab75249)Western blot - EBF1 antibody (ab75249) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab75249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1/312500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.

Sequence similarities

Belongs to the COE family.
Contains 1 IPT/TIG domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9UH73 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • COE1 antibody
  • OLF1 antibody
  • COE1_HUMAN antibody
  • Early B cell factor 1 antibody
  • Early B-cell factor antibody
  • EBF antibody
  • Ebf1 antibody
  • O/E 1 antibody
  • O/E-1 antibody
  • OE-1 antibody
  • Transcription factor COE1 antibody
see all

Database links

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Anti-EBF1 antibody images:

  Western blot - EBF1 antibody (ab75249)

Western blot - EBF1 antibody (ab75249)

Anti-EBF1 antibody (ab75249) at 1 µg/ml + Fetal brain lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 64 kDa
Observed band size : 64 kDa
Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

References for Anti-EBF1 antibody (ab75249)

ab75249 has not yet been referenced specifically in any publications.

Publishing research using ab75249? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"