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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Anti-EBF1 antibody
See all EBF1 products (4) ...
Goat polyclonal to EBF1
This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken
Synthetic peptide: C-HNQLPALANTSV, corresponding to internal sequence amino acids 418-429 of Human EBF1(Peptide available as ab28979.)
C-HNQLPALA NTSV
Human adipose lysate.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
ab85689 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Polyclonal
IgG
Western blot - EBF1 antibody (ab85689)
(enlarge)
Our Abpromise guarantee covers the use of ab85689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
Belongs to the COE family.
Contains 1 IPT/TIG domain.
Nucleus.
Target information above from: UniProt accessionQ9UH73
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - EBF1 antibody (ab85689)

Anti-EBF1 antibody (ab85689) at 0.3 µg/ml + Human adipose lysate (in RIPA buffer) at 35 µg
developed using the ECL technique
Predicted band size : 64 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
ab85689 has not yet been referenced specifically in any publications.
Publishing research using ab85689? Please let us know so that we can cite the reference in this datasheet
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Anti-EBF1 antibody (ab85689) at 0.3 µg/ml + Human adipose lysate (in RIPA buffer) at 35 µg
developed using the ECL technique
Predicted band size : 64 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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