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Western blot - EBF1 antibody (ab85689)
(enlarge)
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Product Name
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EBF1 antibody
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See all EBF1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to EBF1
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Immunogen
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Synthetic peptide: C-HNQLPALANTSV, corresponding to internal sequence amino acids 418-429 of Human EBF1 (NP_076870.1).
BLAST 'C-HNQLPALANTSV' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Chk
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human adipose lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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EBF1 belongs to the COE family. It contains 1 IPT/TIG domain. EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Forms either a homodimer or a heterodimer with a related family member. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab85689 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EBF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EBF1 antibody images, references, products related to ab85689 and other tools.
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EBF1 antibody images:
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 Western blot - EBF1 antibody (ab85689)
EBF1 antibody (ab85689) at 0.3 µg/ml + Human adipose lysate (in RIPA buffer) at 35 µg
developed using the ECL technique
Predicted band size : 64 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
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Search PubMed (MEDLINE) for references to EBF1
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EBF1 antibody - more information
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Customer reviews (feedback) regarding EBF1 antibody |
Customer FAQs regarding EBF1 antibody |
Protocols for EBF1 antibody |
Price and availability of products related to EBF1 antibody |
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EBF1 antibody - related products:
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EBF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about EBF1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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