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Anti-EBF1 antibody (ab85689)

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Overview

Product name

Anti-EBF1 antibody
See all EBF1 products (4) ...

Description

Goat polyclonal to EBF1

Specificity

This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken

Immunogen

Synthetic peptide: C-HNQLPALANTSV, corresponding to internal sequence amino acids 418-429 of Human EBF1(Peptide available as ab28979.)

C-HNQLPALA NTSV

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human adipose lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab85689 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors

  • Western blot - EBF1 antibody (ab85689)Western blot - EBF1 antibody (ab85689) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab85689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.1 - 0.3 µg/ml.Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).

Target

Function

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.

Sequence similarities

Belongs to the COE family.
Contains 1 IPT/TIG domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9UH73 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • COE1 antibody
  • OLF1 antibody
  • COE1_HUMAN antibody
  • Early B cell factor 1 antibody
  • Early B-cell factor antibody
  • EBF antibody
  • EBF1 antibody
  • O/E 1 antibody
  • O/E-1 antibody
  • OE-1 antibody
  • Transcription factor COE1 antibody
see all

Database links

see all

Anti-EBF1 antibody images:

  Western blot - EBF1 antibody (ab85689)

Western blot - EBF1 antibody (ab85689)

Anti-EBF1 antibody (ab85689) at 0.3 µg/ml + Human adipose lysate (in RIPA buffer) at 35 µg
developed using the ECL technique

Predicted band size : 64 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)


Primary incubation was 1 hour.

References for Anti-EBF1 antibody (ab85689)

ab85689 has not yet been referenced specifically in any publications.

Publishing research using ab85689? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"