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Western blot - EBF1 antibody (ab85689)
Western blot - EBF1 antibody (ab85689)
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Product Name 

EBF1 antibody

 

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Product type 

Primary antibodies

Description 

Goat polyclonal to EBF1

Immunogen 

Synthetic peptide: C-HNQLPALANTSV, corresponding to internal sequence amino acids 418-429 of Human EBF1 (NP_076870.1).
BLAST 'C-HNQLPALANTSV' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Chk
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human adipose lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

EBF1 belongs to the COE family. It contains 1 IPT/TIG domain. EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Forms either a homodimer or a heterodimer with a related family member. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.

 

Alternative names EBF1 antibody (ab85689)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

395512    (Chicken)
1879    (Human)
13591    (Mouse)
116543    (Rat)

Omim

    

164343    (Human)

SwissProt

    

Q9UH73    (Human)
Q07802    (Mouse)
Q63398    (Rat)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

ab85689 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EBF1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for EBF1 antibody images, references, products related to ab85689 and other tools.

 

EBF1 antibody images:

Product image  Western blot - EBF1 antibody (ab85689)

Western blot - EBF1 antibody (ab85689)

EBF1 antibody (ab85689) at 0.3 µg/ml + Human adipose lysate (in RIPA buffer) at 35 µg
developed using the ECL technique

Predicted band size : 64 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)


Primary incubation was 1 hour.

 
 

Search PubMed (MEDLINE) for references to EBF1

 

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