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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Anti-EBF2 antibody
See all EBF2 products (2) ...
Rabbit polyclonal to EBF2
WB, ELISAmore details
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
A region within synthetic peptide: IAEALYSVPR NPSQLPALSS SPAHSGMMGI NSYGSQLGVS ISESTQGNNQ, corresponding to C terminal amino acids 397-446 of Human EBF2
IAEALYSVPR NPSQLPALSS SPAHSGMMGI NSYGSQLGVS ISESTQGNNQ
HepG2 cell lysate.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
Polyclonal
IgG
Western blot - EBF2 antibody (ab56048)
(enlarge)
Our Abpromise guarantee covers the use of ab56048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. (ELISA titre using peptide based assay: 1/312500)
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
EBF2 (Early B-cell factor 2) belongs to the conserved Olf/EBF family of helix-loop-helix transcription factors. In osteoblasts it activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. EBF2 acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. It recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
Nuclear
Western blot - EBF2 antibody (ab56048)

Anti-EBF2 antibody (ab56048) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 63 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
ab56048 has not yet been referenced specifically in any publications.
Publishing research using ab56048? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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Anti-EBF2 antibody (ab56048) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 63 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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