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Anti-EBF2 antibody (ab56048)

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Overview

Product name

Anti-EBF2 antibody
See all EBF2 products (2) ...

Description

Rabbit polyclonal to EBF2

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow

Immunogen

A region within synthetic peptide: IAEALYSVPR NPSQLPALSS SPAHSGMMGI NSYGSQLGVS ISESTQGNNQ, corresponding to C terminal amino acids 397-446 of Human EBF2

IAEALYSVPR NPSQLPALSS SPAHSGMMGI NSYGSQLGVS ISESTQGNNQ

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HepG2 cell lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Olfactory system
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH

  • Western blot - EBF2 antibody (ab56048)Western blot - EBF2 antibody (ab56048) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab56048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. (ELISA titre using peptide based assay: 1/312500)
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

EBF2 (Early B-cell factor 2) belongs to the conserved Olf/EBF family of helix-loop-helix transcription factors. In osteoblasts it activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. EBF2 acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. It recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.

Cellular localization

Nuclear

Alternative names

  • COE 2 antibody
  • COE2 antibody
  • Collier Olf and EBF 2 antibody
  • Early B cell factor 2 antibody
  • EBF 2 antibody
  • FLJ11500 antibody
  • Metencephalon mesencephalnon olfactory transcription factor 1 antibody
  • O/E 3 antibody
  • O/E3 antibody
  • OE 3 antibody
  • OE3 antibody
  • OLF 1/EBF like 3 antibody
  • Transcription factor COE2 antibody
see all

Database links

Anti-EBF2 antibody images:

  Western blot - EBF2 antibody (ab56048)

Western blot - EBF2 antibody (ab56048)

Anti-EBF2 antibody (ab56048) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 63 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)


Gel concentration: 12%

References for Anti-EBF2 antibody (ab56048)

ab56048 has not yet been referenced specifically in any publications.

Publishing research using ab56048? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"