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Western blot - EBF2 antibody (ab56048)
(enlarge)
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Product Name
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EBF2 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EBF2
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Immunogen
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A region within synthetic peptide: IAEALYSVPR NPSQLPALSS SPAHSGMMGI NSYGSQLGVS ISESTQGNNQ, corresponding to C terminal amino acids 397-446 of Human EBF2
BLAST 'IAEALYSVPR NPSQLPALSS SPAHSGMMGI NSYGSQLGVS ISESTQGNNQ ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. (ELISA titre using peptide based assay: 1/312500)
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Olfactory system
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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EBF2 (Early B-cell factor 2) belongs to the conserved Olf/EBF family of helix-loop-helix transcription factors. In osteoblasts it activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. EBF2 acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. It recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EBF2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EBF2 antibody images, references, products related to ab56048 and other tools.
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EBF2 antibody images:
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 Western blot - EBF2 antibody (ab56048)
EBF2 antibody (ab56048) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 63 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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Search PubMed (MEDLINE) for references to EBF2
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EBF2 antibody - more information
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Customer reviews (feedback) regarding EBF2 antibody |
Customer FAQs regarding EBF2 antibody |
Protocols for EBF2 antibody |
Price and availability of products related to EBF2 antibody |
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EBF2 antibody - related products:
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EBF2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about EBF2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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