Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Anti-EBF3 antibody (ab77602) at 5 µg/ml + immunogen (tagged 100 aa recombinant fragment) at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size : 65 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
MW of protein tag alone: 26 kDa
Anti-EBF3 antibody (ab77602) at 5 µg/ml + IMR-32 cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size : 65 kDa
Observed band size : 65 kDa
Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.
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