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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-EBF3 antibody
See all EBF3 products (4) ...
Description
Mouse monoclonal to EBF3
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Horse, Chicken, Dog, Pig, Xenopus laevis, Chimpanzee
Immunogen
Recombinant fragment: LYGMPHNNQE IILKRAADIA EALYSVPRNH NQIPTLGNNP AHTGMMGVNS FSSQLAVNVS ETSQANDQVG YSRNTSSVSP RGYVPSSTPQ QSNYNTVSTS, corresponding to amino acids 381-480 of Human EBF3 (NP_001005463), with a proprietary tag.
LYGMPHNNQE IILKRAADIA EALYSVPRNH NQIPTLGNNP AHTGMMGVNS FSSQLAVNVS ETSQANDQVG YSRNTSSVSP RGYVPSSTPQ QSNYNTVSTS
Positive control
IMR-32 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 65 kDa.
ELISA: Use at an assay dependent dilution.
Target
Function
Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
Tissue specificity
Expressed in brain.
Sequence similarities
Belongs to the COE family.
Contains 1 IPT/TIG domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9H4W6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- COE 3 antibody
- COE3 antibody
- COE3_HUMAN antibody
see all
Database links
- Entrez Gene: 253738 Human
- Entrez Gene: 13593 Mouse
- Entrez Gene: 361668 Rat
- Entrez Gene: 399125 Xenopus laevis
- Omim: 607407 Human
- SwissProt: Q9H4W6 Human
- SwissProt: O08791 Mouse
- SwissProt: O73742 Xenopus laevis
see all
Anti-EBF3 antibody images:
Western blot - EBF3 antibody (ab77602)
Anti-EBF3 antibody (ab77602) at 5 µg/ml + immunogen (tagged 100 aa recombinant fragment) at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size : 65 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
MW of protein tag alone: 26 kDa
Western blot - EBF3 antibody (ab77602)
Anti-EBF3 antibody (ab77602) at 5 µg/ml + IMR-32 cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size : 65 kDa
Observed band size : 65 kDa
Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.
References for Anti-EBF3 antibody (ab77602)
ab77602 has not yet been referenced specifically in any publications.
Publishing research using ab77602? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"