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Lanes 1 - 3 : Anti-EBI3 antibody (ab72330) at 2 µg/ml
Lane 4 : Anti-EBI3 antibody (ab72330) at 5 µg/ml
Lane 1 : Daudi cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Ramos cell lysate with immunizing peptide
Lane 4 : RAW cell lysate
Predicted band size : 25 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Anti-EBI3 antibody (ab72330) at 2 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 8 minutes
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