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Products:Immunology >> Innate Immunity >> Cytokines >> Interleukins
Overview
Product name
Anti-EBI3 antibody
See all EBI3 products (8) ...
Description
Rabbit polyclonal to EBI3
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to a region between residues 50-100 of human EBI3 (NP_005746).
Positive control
Lysates from Daudi, Ramos or RAW cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> Cytokines >> Interleukins
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Western blot - EBI3 antibody (ab72330)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 - 6 µg/ml.Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
Target
Function
Cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
Sequence similarities
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 2 fibronectin type-III domains.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ14213
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytokine receptor antibody
- EBI3 antibody
- EBV induced gene 3 protein antibody
see all
Database links
- Entrez Gene: 10148 Human
- Entrez Gene: 50498 Mouse
- Omim: 605816 Human
- SwissProt: Q14213 Human
- SwissProt: O35228 Mouse
- Unigene: 501452 Human
- Unigene: 256798 Mouse
Anti-EBI3 antibody images:
Western blot - EBI3 antibody (ab72330)
Lanes 1 - 3 : Anti-EBI3 antibody (ab72330) at 2 µg/ml
Lane 4 : Anti-EBI3 antibody (ab72330) at 5 µg/ml
Lane 1 : Daudi cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Ramos cell lysate with immunizing peptide
Lane 4 : RAW cell lysate
Predicted band size : 25 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
References for Anti-EBI3 antibody (ab72330)
ab72330 has not yet been referenced specifically in any publications.
Publishing research using ab72330? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"