![Western blot - EBP50 antibody [EBP-10] (ab9526) image](#EBP50-Primary-antibodies-ab9526-2.jpg)
 image](#EBP50-Primary-antibodies-ab9526-3.jpg)
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Product name
Anti-EBP50 antibody [EBP-10]
See all EBP50 products (7) ...
Description
Mouse monoclonal [EBP-10] to EBP50
Specificity
Human ERM (ezrin/radixin/moesin)-binding phosphoprotein of 50 kDa (EBP50) / Na+/H+ exchange regulatory factor (NHERF)
Tested applications
Flow Cyt, IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Positive control
RAJI human lymphoma cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Clonality
Monoclonal
Clone number
EBP-10
Isotype
IgG2b
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells.
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration. Recommended dilution: 2µg/ml. Sample preparation: Lysis buffer with maltoside (1%). Application note: Non-reducing conditions with SDS-PAGE (10% separating gel).
Target
Function
Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.
Tissue specificity
Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
Involvement in disease
Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).
Sequence similarities
Contains 2 PDZ (DHR) domains.
Post-translational
modifications
Phosphorylated on serine residues.
Cellular localization
Cytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
Target information above from: UniProt accessionO14745
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EBP 50 antibody
- EBP50 antibody
- Ezrin radixin moesin binding phosphoprotein 50 antibody
see all
Database links
- Entrez Gene: 9368 Human
- Omim: 604990 Human
- SwissProt: O14745 Human
- Unigene: 724482 Human
- Unigene: 728760 Human
Anti-EBP50 antibody [EBP-10] images:
Western blot - EBP50 antibody [EBP-10] (ab9526)
![Western blot - EBP50 antibody [EBP-10] (ab9526)](/ps/datasheet/images/9/ab9526/EBP50-Primary-antibodies-ab9526-2.jpg)
All lanes : Anti-EBP50 antibody [EBP-10] (ab9526) at 2 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Flow Cytometry-Anti-EBP50 antibody [EBP-10](ab9526)
](/ps/datasheet/images/9/ab9526/EBP50-Primary-antibodies-ab9526-3.jpg)
Overlay histogram showing HepG2 cells stained with ab9526 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9526, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-EBP50 antibody [EBP-10] (ab9526)
This product has been referenced in:
- Reining SC et al. GABARAP deficiency modulates expression of NaPi-IIa in renal brush-border membranes. Am J Physiol Renal Physiol 296:F1118-28 (2009).Read more (PubMed: 19225049) »
- Saotome I et al. Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine. Dev Cell 6:855-64 (2004).Read more (PubMed: 15177033) »
See all 4 publications for this product
Publishing research using ab9526? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"