EBV Early Antigen Diffuse Ea-D antibody [8.F.92] (ab30541)

Product Name 

EBV Early Antigen Diffuse Ea-D antibody [8.F.92]

Product type 

Primary antibodies

Description 

Mouse monoclonal [8.F.92] to EBV Early Antigen Diffuse Ea-D

Immunogen 

Epstein Barr Virus Early Diffuse Protein (Ea-D) p52/50.

Species Reactivity 

Reacts with Epstein-Barr Virus.
Not yet tested in other species.

Specificity 

Ab30541 recognizes Early Diffuse Protein (Ea-D) p52/50 of Epstein Barr Virus. The antibody is reactive in IFA of infected cells, immunoprecipitation of specific viral protein and western blot and it recognizes does recognize strain B98-8 of EBV strain.

Tested applications 
(see key)

ELISA, ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Epstein Barr

Relevance 

The EBV Early Diffuse Antigen Diffuse EA-D or EBV DNA polymerase accessory protein, is an essential component of the viral DNA polymerase and is required for lytic EBV replication. In addition to its polymerase accessory protein function, it has recently been reported that Ea-D is a transcriptional activator, inducing expression of the essential oriLyt promoter, BHLF1.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

8.F.92

Isotype 

IgG2

Purity 

IgG fraction

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 40% Glycerol, PBS, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

Ammonium sulfate fractionation and column chromatography.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

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