![Immunocytochemistry/ Immunofluorescence - EBV Early Antigen Diffuse Ea-D antibody [G3-E31] (ab49668) image](#ab49668_1.jpg)
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Products:Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Epstein Barr
Overview
Product name
Anti-EBV Early Antigen Diffuse Ea-D antibody [G3-E31]
See all EBV Early Antigen Diffuse Ea-D products (2) ...
Description
Mouse monoclonal [G3-E31] to EBV Early Antigen Diffuse Ea-D
Specificity
This antibody is specific to EBV Early Antigen Diffuse Ea-D encoded by the BMRF-1 open reading frame.
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with Epstein Barr virus (EBV) Not yet tested in other species.
Immunogen
Lysates of B95-8 cells treated with phorbol myristic acid (PMA).
Positive control
Immunocytochemistry: Any cell line with productive EBV infection; Raji cells stimulated with PMA. Western Blotting: B95-8 cell line.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
G3-E31
Myeloma
P3x63-Ag8.653
Isotype
IgG1
Research areas
Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Epstein Barr
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Immunocytochemistry/ Immunofluorescence - EBV Early Antigen Diffuse Ea-D antibody [G3-E31] (ab49668)
![Immunocytochemistry/ Immunofluorescence - EBV Early Antigen Diffuse Ea-D antibody [G3-E31] (ab49668) image](/ps/datasheet/Images/49/ab49668/ab49668_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab49668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
- 1 Image
ICC/IF
IHC-P
Application notes
ICC/IF: 1/200 - 1/400.
WB: 1/250 - 1/500. Predicted molecular weight: 50 - 52 kDa.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. This antibody gives excellent staining of formalin-fixed, paraffin-embedded B95-8 and Raji cell pellets but has not been fully evaluated on clinical material.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
The EBV Early Diffuse Antigen Diffuse EA-D or EBV DNA polymerase accessory protein, is an essential component of the viral DNA polymerase and is required for lytic EBV replication. In addition to its polymerase accessory protein function, it has recently been reported that Ea-D is a transcriptional activator, inducing expression of the essential oriLyt promoter, BHLF1.
Cellular localization
Nuclear
Alternative names
- BMRF1 antibody
- DNA polymerase accessory protein antibody
- Early antigen protein D antibody
see all
Anti-EBV Early Antigen Diffuse Ea-D antibody [G3-E31] images:
Immunocytochemistry/ Immunofluorescence - EBV Early Antigen Diffuse Ea-D antibody [G3-E31] (ab49668)
Ab49668, at a dilution of 1/200, staining paraffin embedded Epstein-Barr virus transformed B95-8 cells by Immunocytochemistry. Note intense staining of infected cells only where early virus replication is underway.
References for Anti-EBV Early Antigen Diffuse Ea-D antibody [G3-E31] (ab49668)
This product has been referenced in:
- Thorne LBet al. Real-time PCR measures Epstein-Barr Virus DNA in archival breast adenocarcinomas. Diagn Mol Pathol 14:29-33 (2005).Read more (PubMed: 15714061) »
See 1 publication for this product
Publishing research using ab49668? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"