EBV Early Antigen Diffuse Ea-D antibody [G3-E31] (ab49668)

Product Name 

EBV Early Antigen Diffuse Ea-D antibody [G3-E31]

Product type 

Primary antibodies

Description 

Mouse monoclonal [G3-E31] to EBV Early Antigen Diffuse Ea-D

Immunogen 

Lysates of B95-8 cells treated with phorbol myristic acid (PMA).

Species Reactivity 

Reacts with Epstein Barr virus (EBV)
Not yet tested in other species.

Specificity 

This antibody is specific to EBV Early Antigen Diffuse Ea-D encoded by the BMRF-1 open reading frame.

Tested applications 
(see key)

ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/200 - 1/400.
WB: 1/250 - 1/500. Predicted molecular weight: 50 - 52 kDa.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. This antibody gives excellent staining of formalin-fixed, paraffin-embedded B95-8 and Raji cell pellets but has not been fully evaluated on clinical material.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Immunocytochemistry: Any cell line with productive EBV infection; Raji cells stimulated with PMA.
Western Blotting: B95-8 cell line.

Cellular localization 

Nuclear

Research areas 

Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Epstein Barr

Relevance 

The EBV Early Diffuse Antigen Diffuse EA-D or EBV DNA polymerase accessory protein, is an essential component of the viral DNA polymerase and is required for lytic EBV replication. In addition to its polymerase accessory protein function, it has recently been reported that Ea-D is a transcriptional activator, inducing expression of the essential oriLyt promoter, BHLF1.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

G3-E31

Myeloma 

P3x63-Ag8.653

Isotype 

IgG1

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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References for EBV Early Antigen Diffuse Ea-D antibody [G3-E31] (ab49668)

  This clone has been used in:

Thorne LB et al. Real-time PCR measures Epstein-Barr Virus DNA in archival breast adenocarcinomas. Diagn Mol Pathol 14:29-33 (2005). PubMed: 15714061

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