Anti-EBV Early Antigen Restricted Ea-R p17 antibody [2Q1912] (ab64281)

Hi Tech,

Can you recommend anything for my customer who intended to do Elisa.

She also wanted to know the most sensitive way to detect her samples as she

suspects the cell has little of the protein.

Kindly reply the soonest.

Thanks

ANSWER:

Thank you for your inquiry.

In case the customer would like to perform a sandwich ELISA (sELISA) for the EBV viral capsid antigen, I would suggest to test the antibody clones [2E08] and [8.F.90] (ab48414 and ab32803, see below). To our knowledge, this pair has not yet been tested in sELISA, and therefore, I can offer a discount off a future purchase if the customer buys them now, test them in sELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive the feedback. The discount would be to the value of 1 free primary antibody each. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Please let me know if the customer is interested in this offer and I would be happy to help you further.

If these antibodies are used in sELISA, one of them (the detection antibody) needs to be conjugated to an enzyme such as HRP and we would therefore recommend our EasyLink conjugation kits ab102889 or ab102890 (www.abcam.com/EasyLink or see below). If the customer is interested in one of these, I would be pleased to offer a discount.

Unfortunately, we do not have any other antibodies available against EBV Ea-R p17 than ab64281 and against EBV Ribonucleotide Reductase than ab6504, respectively. Therefore, I cannot give a recommendation for a suitable paired antibody to use in sELISA. Having said this, I could recommend the HRP-conjugated secondary antibodies ab98693 (for the mouse-IgG1 antibody ab6504) and ab98698 (for the mouse-IgG2a antibody ab64281) in case the customer would like to set up a conventional indirect ELISA (see below). Using a secondary antibody amplifies the signal and this might be useful for proteins of low abundance.

http://www.abcam.com/index.html?datasheet=32803 http://www.abcam.com/index.html?datasheet=32803.

http://www.abcam.com/index.html?datasheet=102889 http://www.abcam.com/index.html?datasheet=102889.

http://www.abcam.com/index.html?datasheet=102890 http://www.abcam.com/index.html?datasheet=102890.

http://www.abcam.com/index.html?datasheet=98693 http://www.abcam.com/index.html?datasheet=98693.

http://www.abcam.com/index.html?datasheet=98698 http://www.abcam.com/index.html?datasheet=98698.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Hi Tech, I have a customer who has previously bought 3 antibodies to do WB and now wants to do it for ELISA. Could you suggest any suitable Elisa Kit for the application? She is using Crude Cell lysate to study expression of the below EBV antigens in B95-8 and P3HR-1 cell lines using ELISA assay. The antibodies they are currently using are AB6504, AB64281 and AB30542. Hope to receive your prompt reply. Thanks.

ANSWER:

Thank you for your inquiry.

Unfortunately, we do not have suitable ELISA kits available to detect the mentioned EBV antigens.

However, I am pleased to let you know that ab6504 and ab64281 are tested and guaranteed to work in ELISA. Therefore, the customer could set up her own ELISA, and I have attached our protocols for more detailed information, which can also be found on our protocols website (http://www.abcam.com/index.html?pageconfig=popular_protocols).

As ab30542 is currently not available, I may suggest ab48414 as alternative which is also guaranteed to work in ELISA: http://www.abcam.com/index.html?datasheet=48414 http://www.abcam.com/index.html?datasheet=48414.

If the customer decides to perform a Sandwich ELISA which might be suitable for using crude cell lysate as the sample does not have to be purified, and the assay can be very sensitive, I would be pleased to assist her in finding the most suitable pair of antibodies. Also, she may be eligible for our testing discount program if the antibodies have not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.