Anti-EBV Latent Membrane Protein 1 antibody [CS1, CS2, CS3, CS4], prediluted (ab7502)
Key features and details
- Mouse monoclonal [CS1, CS2, CS3, CS4] to EBV Latent Membrane Protein 1, prediluted
- Suitable for: IHC-P, IHC-Fr
- Isotype: IgG1
Overview
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Product name
Anti-EBV Latent Membrane Protein 1 antibody [CS1, CS2, CS3, CS4], prediluted
See all EBV Latent Membrane Protein 1 primary antibodies -
Description
Mouse monoclonal [CS1, CS2, CS3, CS4] to EBV Latent Membrane Protein 1, prediluted -
Host species
Mouse -
Specificity
This antibody is specific to 60 kD latent membrane protein (LMP1) encoded by the BNLF1 gene of the EBV. Each clone reacts with different epitopes on the hydrophilic c-terminus of the cytoplasmic domain of LMP1. This antibody stains strongly with EBV positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis. -
Tested applications
Suitable for: IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Epstein-Barr virus -
Immunogen
Recombinant fusion protein containing sequences of bacterial beta-galactosidase and EBV encoded Latent membrane protein.
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Positive control
- Infected lymphoblastoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.3
Constituent: PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
This product is supplied as a tissue culture supernatant and contains sodium azide as a preservative. -
Clonality
Monoclonal -
Clone number
CS1, CS2, CS3, CS4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Relevance
EBV is a human herpesvirus that establishes a life-long persistence in the host. The virus infects the vast majority of the world's adult population and is well known for its association with a broad spectrum of benign and malignant diseases, including infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal carcinoma, and is causally associated with lymphoid and epithelial malignancies, including post-transplant lymphoproliferative disorders, Hodgkin's disease, anaplastic nasopharyngeal carcinoma and gastric carcinomas. Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is a transforming protein that affects multiple cell signalling pathways and contributes to EBV-associated oncogenesis. LMP1 can be expressed in some states of EBV latency, and significant induction of full-length LMP1 is also observed frequently during virus reactivation into the lytic cycle. LMP1 is critical for EBV-infected cell activation, adhesion and survival, and is usually expressed in the malignant cells. -
Database links
- Entrez Gene: 3783750 Epstein-Barr virus
- SwissProt: P0C741 Epstein-Barr virus
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Alternative names
- BNLF1 antibody
- EBV antibody
- Epstein Barr virus antibody
see all
Images
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The image shows lung tissue diagnosed with Post-Transplantation Lymphoproliferative Disorder (PTLD). Mononuclear cells are stained brown with DAB chromogen around the cytoplasm - typical for Latent Membrane Protein-1 of EBV.
This picture was kindly supplied as part of the review submitted by Pradeep Rai.
Datasheets and documents
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Datasheet download
References (2)
ab7502 has been referenced in 2 publications.
- Li C et al. EBNA2-deleted Epstein-Barr virus (EBV) isolate, P3HR1, causes Hodgkin-like lymphomas and diffuse large B cell lymphomas with type II and Wp-restricted latency types in humanized mice. PLoS Pathog 16:e1008590 (2020). PubMed: 32542010
- Nawandar DM et al. Differentiation-Dependent LMP1 Expression Is Required for Efficient Lytic Epstein-Barr Virus Reactivation in Epithelial Cells. J Virol 91:N/A (2017). PubMed: 28179525