Overview

  • Product nameECH1 Human Profiling ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    HepG2 3 < 8.4%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich (qualitative)
  • Sensitivity
    > 0.3 µg/ml
  • Range
    8 µg/ml - 500 µg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Utilization of fatty acids as energy source is a fundamental process in all mammals and involves the evolutionarily conserved ß-oxidation pathway of acyl CoAs, which occurs in mitochondria and peroxisomes. The metabolism of saturated fatty acids requires four enzyme activities which are intrinsic to the ß-oxidation pathway. However, the metabolism of ubiquitously occurring unsaturated fatty acids requires auxiliary enzymes in addition to the spiral of the four ß-oxidation enzymes. These auxiliary activities consist of Delta3,5, Delta2,4-dienoyl CoA isomerase (i.e. ECH1), Delta2,4 dienoyl CoA isomerase, Delta2,4-dienoyl CoA reductase and Delta3, Delta2 - enoyl CoA isomerase.

     

    ab133983 ECH1  Profiling ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of ECH1 protein in cell and tissue lysates from human, rat and mouse. The assay employs an antibody specific for ECH1 protein coated onto well plate strips. Samples are pipetted into the wells and ECH1 present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a biotin labelled anti-ECH1 detector antibody is added. After washing away unbound detector antibody, HRP-conjugated streptavidin specific for the biotin labeled detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of ECH1 bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

     

  • Notes

    Determined minimum detectable dose is 8 µg/mL when using rat heart homogenate or HepG2 whole cell lysates.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

ECH1 Human Profiling ELISA Kit images

  • This ELISA kit is able to quantify the endogenous ECH1 level as sensitive as Western blot but with the advantage of high throughput. (A) 25µL of HepG2 or HDFn was loaded on a gel and transferred to PVDF membrane following Abcam’s standard western blot protocol. Samples were then blotted with an ECH1 antibody. (B) At the meantime, same amount of cell extracts were captured and measured by ECH1 ELISA kit.
  • Example of a serially titrated control curve of HepG2 cells.

Protocols

References for ECH1 Human Profiling ELISA Kit (ab133983)

ab133983 has not yet been referenced specifically in any publications.

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