![Western blot - Anti-ECH1 antibody [3B7CF2AC8] (ab123450) image](#ECH1-Primary-antibodies-ab123450-1.gif)
![Immunocytochemistry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450) image](#ECH1-Primary-antibodies-ab123450-2.gif)
![Flow Cytometry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450) image](#ECH1-Primary-antibodies-ab123450-3.gif)
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Product name
Anti-ECH1 antibody [3B7CF2AC8]
See all ECH1 products (4) ...
Description
Mouse monoclonal [3B7CF2AC8] to ECH1
Tested applications
WB, IP, In-Cell ELISA, ICC, Flow Cytmore details
Cross reactivity
Reacts with
Rat, Cow, Human
Immunogen
Purified Human liver mitochondria.
Positive control
HDFn cells; Human, Rat and Cow heart homogenates; Human liver homogenate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HBS
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab123450 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
3B7CF2AC8
Isotype
IgG2b
Light chain type
kappa
Research areas
Metabolism >> Metabolic signalling pathways >> Lipid and lipoprotein metabolism >> Fatty acids
Metabolism >> Mitochondrial Metabolism >> Mitochondrial markers
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab123450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 36 kDa.
IP: Use at an assay dependent concentration.
In-Cell ELISA: Use a concentration of 1 µg/ml
ICC: Use a concentration of 1 µg/ml
-
Flow Cyt: Use a concentration of 1 µg/ml
Target
Function
Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
Pathway
Lipid metabolism; fatty acid beta-oxidation.
Sequence similarities
Belongs to the enoyl-CoA hydratase/isomerase family.
Cellular localization
Mitochondrion. Peroxisome.
Target information above from: UniProt accessionQ13011
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- 4)-dienoyl-CoA isomerase antibody
- 5)-Delta(2 antibody
see all
Database links
- Entrez Gene: 507431 Cow
- Entrez Gene: 1891 Human
- Entrez Gene: 64526 Rat
- Omim: 600696 Human
- SwissProt: Q13011 Human
- SwissProt: Q62651 Rat
- Unigene: 196176 Human
- Unigene: 6148 Rat
Anti-ECH1 antibody [3B7CF2AC8] images:
Western blot - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
![Western blot - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)](/ps/datasheet/images/123/ab123450/ECH1-Primary-antibodies-ab123450-1.gif)
All lanes : Anti-ECH1 antibody [3B7CF2AC8] (ab123450) at 1 µg/ml
Lane 1 : Human heart homogenate
Lane 2 : Human liver homogenate
Lane 3 : Rat heart homogenate
Lane 4 : Cow heart homogenate
Lysates/proteins at 10 µg/ml per lane.
Secondary
Goat-anti-Mouse HRP at 3000
Predicted band size : 36 kDa
Immunocytochemistry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
![Immunocytochemistry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)](/ps/datasheet/images/123/ab123450/ECH1-Primary-antibodies-ab123450-2.gif)
ab123450 at 1µg/ml staining ECH1 in 4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized HDFn cells by Immunocytochemistry followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).
Flow Cytometry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
![Flow Cytometry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)](/ps/datasheet/images/123/ab123450/ECH1-Primary-antibodies-ab123450-3.gif)
ab123450 at 1µg/ml staining ECH1 in HDFn cells by Flow Cytometry (blue) or no primary antibody control (red).
References for Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
ab123450 has not yet been referenced specifically in any publications.
Publishing research using ab123450? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"