![Flow Cytometry - ECH1 antibody [9D11AF3 ] (ab110294) image](#ECH1-Primary-antibodies-ab110294-2.jpg)
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Product name
Anti-ECH1 antibody [9D11AF3 ]
See all ECH1 products (4) ...
Description
Mouse monoclonal [9D11AF3 ] to ECH1
Tested applications
ICC/IF, In-Cell ELISA, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
human liver mitochondria
Positive control
HDFn cells and HL-60 cells
General notes
ECH1 is a member of the hydratase/isomerase superfamily involved in fatty acid metabolism. It is localized to peroxisomes and/or mitochondria in different mammals. In humans, levels of ECH1 are modulated by peroxisome proliferators. As the name implies, ECH1 catalyzes the isomerization of cis ?3,5, double bonds in unsaturated acyl-CoAs to yield trans ?2,4-dienoyl CoAs. These are substrates for ?2,4-dienoyl CoA reductase which produces trans ?3 enoyl CoA. This intermediate can then be further transformed by ?3, ?2 - enoyl CoA isomerase to finally yield the trans-2 double bond configured fatty acyl CoA, which can be then directed into the ß-oxidation pathway. Expression of ECH1 is highest in metabolically active tissues such as heart, skeletal muscle, central nervous system, liver and kidney. Recent studies show that it is down regulated in gastric carcinoma and CLL.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110294 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
9D11AF3
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Fatty acids
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 4 µg/ml.
In-Cell ELISA: Use a concentration of 4 µg/ml.
Flow Cyt: Use a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
Target
Function
Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
Pathway
Lipid metabolism; fatty acid beta-oxidation.
Sequence similarities
Belongs to the enoyl-CoA hydratase/isomerase family.
Cellular localization
Mitochondrion. Peroxisome.
Target information above from: UniProt accessionQ13011
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- 4)-dienoyl-CoA isomerase antibody
- 5)-Delta(2 antibody
see all
Database links
- Entrez Gene: 1891 Human
- Entrez Gene: 64526 Rat
- Omim: 600696 Human
- SwissProt: Q13011 Human
- SwissProt: Q62651 Rat
- Unigene: 196176 Human
- Unigene: 6148 Rat
Anti-ECH1 antibody [9D11AF3 ] images:
Flow Cytometry - ECH1 antibody [9D11AF3 ] (ab110294)
![Flow Cytometry - ECH1 antibody [9D11AF3 ] (ab110294)](/ps/datasheet/images/110/ab110294/ECH1-Primary-antibodies-ab110294-2.jpg)
HL-60 cells were stained with 1 µg/mL ab110294 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Immunocytochemistry - Anti-ECH1 antibody [9D11AF3 ] (ab110294)
![Immunocytochemistry - Anti-ECH1 antibody [9D11AF3 ] (ab110294)](/ps/datasheet/images/110/ab110294/ECH1-Primary-antibodies-ab110294-6.tif)
Immunocytochemistry image of ab110294 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (9D11AF3, 4 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the mitochondria.
References for Anti-ECH1 antibody [9D11AF3 ] (ab110294)
ab110294 has not yet been referenced specifically in any publications.
Publishing research using ab110294? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"