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Western blot - ECHS1 antibody (ab84650)
Western blot - ECHS1 antibody (ab84650)
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Product Name 

ECHS1 antibody

 

See all ECHS1 antibodies (3)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ECHS1

Immunogen 

Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (IIAEKRGKNN TVGLIQLNRP KALNALCDGL IDELNQALKT FEEDPAVGAI) of human ECHS1 (NP_004083).
BLAST 'IIAEKRGKNN TVGLIQLNRP KALNALCDGL IDELNQALKT FEEDPAVGAI ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human fetal heart lysate.

Cellular localization 

Mitochondrion matrix.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria

Relevance 

ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway.

 

Alternative names ECHS1 antibody (ab84650)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1892    (Human)
93747    (Mouse)
140547    (Rat)

Omim

    

602292    (Human)

SwissProt

    

P30084    (Human)
Q8BH95    (Mouse)
P14604    (Rat)

Unigene

    

76394    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Purification notes 

ab84650 is purified by a peptide affinity chromatography method.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ECHS1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ECHS1 antibody images, references, products related to ab84650 and other tools.

 

ECHS1 antibody images:

Product image  Western blot - ECHS1 antibody (ab84650)

Western blot - ECHS1 antibody (ab84650)

ECHS1 antibody (ab84650) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal heart lysate at 10 µg

Secondary
anti-Rabbit IgG HRP at 1/50000 dilution

Predicted band size : 28 kDa
Observed band size : 28 kDa

 
 

Search PubMed (MEDLINE) for references to ECHS1

 

ECHS1 antibody - more information

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ECHS1 antibody - related products:

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Other ECHS1 Antibodies

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ECHS1 antibody - other tools:

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