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Western blot - ECHS1 antibody (ab89126)
Western blot - ECHS1 antibody (ab89126)
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Immunocytochemistry/ Immunofluorescence - ECHS1 antibody (ab89126)
Immunocytochemistry/ Immunofluorescence - ECHS1 antibody (ab89126)
(enlarge)


Product Name 

ECHS1 antibody

 

See all ECHS1 antibodies (2)...

Product type 

Primary antibodies

Description 

Mouse polyclonal to ECHS1

Immunogen 

Full length Human ECHS1 (NP_004083.2, amino acids 1-290).

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC/IF, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human liver lysate, ECHS1 transfected 293T cell lysate, HeLa cells.

Cellular localization 

Mitochondrion matrix.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria

Relevance 

ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway.

 

Alternative names ECHS1 antibody (ab89126)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1892    (Human)

Omim

    

602292    (Human)

SwissProt

    

P30084    (Human)

Unigene

    

76394    (Human)

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 1X PBS, pH 7.2

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ECHS1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ECHS1 antibody images, references, products related to ab89126 and other tools.

 

ECHS1 antibody images:

Product image  Western blot - ECHS1 antibody (ab89126)

Western blot - ECHS1 antibody (ab89126)

ECHS1 antibody (ab89126) at 1 µg/ml + Human liver lysate at 50 µg

Predicted band size : 31 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)

 

Product image  Immunocytochemistry/ Immunofluorescence - ECHS1 antibody (ab89126)

Immunocytochemistry/ Immunofluorescence - ECHS1 antibody (ab89126)

ab89126, at 10 µg/ml, staining ECHS1 in HeLa cells by Immunofluorescence.

 
 

Search PubMed (MEDLINE) for references to ECHS1

 

ECHS1 antibody - more information

Customer reviews (feedback) regarding ECHS1 antibody

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ECHS1 antibody - related products:

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