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ECL Western Blotting Substrate Kit - 50 Tests (ab65623)

Q&A (4)Specific References (2)

Overview

  • Product nameECL Western Blotting Substrate Kit - 50 TestsSee all ECL kits ...
  • Product overview

    The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.

     

    Looking for our new high sensitivity Optiblot ECL kits. View them here.

  • Notes

    Reagents provided are sufficient for 400 cm2 of membrane.

  • Tested applicationsWB more details

Properties

  • RelevanceECL (enhanced luminol-based chemiluminescent) detection is a sensitive, nonradioactive method for easy detection of horseradish peroxidase (HRP) on western blots.
    • Alternative names
        Enhanced luminol based chemiluminescent

    Applications

    Our Abpromise guarantee covers the use of ab65623 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB

    ECL Western Blotting Substrate Kit - 50 Tests images

    • - ECL Western Blotting Substrate Kit - 50 Tests (ab65623)
      - ECL Western Blotting Substrate Kit - 50 Tests (ab65623)
      ECL Western Blotting Substrate

    References for ECL Western Blotting Substrate Kit - 50 Tests (ab65623)

    This product has been referenced in:
    • Leeming DJ  et al. Enzyme-linked immunosorbent serum assays (ELISAs) for rat and human N-terminal pro-peptide of collagen type I (PINP)--assessment of corresponding epitopes. Clin Biochem 43:1249-56 (2010). Read more (PubMed: 20709044) »
    • Mohan S  et al. Typhonium flagelliforme induces apoptosis in CEMss cells via activation of caspase-9, PARP cleavage and cytochrome c release: its activation coupled with G0/G1 phase cell cycle arrest. J Ethnopharmacol 131:592-600 (2010). WB . Read more (PubMed: 20673794) »

    See all 2 Publications for this product

    Publishing research using ab65623 ? Please let us know so that we can cite the reference in this datasheet

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    Displaying 1 - 4 of 4 results for Abreviews and Q&A

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    Do you happen to have a product insert for this product? I have emailed the customer the protocol but they would like to know;

    If you can get me the product insert so I can check, for example, the area of blots that can be done by this product (5...

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    Thank you for contacting us.

    I am sorry do you need protcol or datasheet? The datasheet is attached.

    http://www.abcam.com/ECL-Western-Blotting-Substrate-Kit-50-Tests-ab65623.html

    I hope this information is helpful to you. Please...

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    She is looking for suitable WB kit with peroxidase or phosphatase substrate.
    We suggested: ECL WB Substrate Kit #ab65623.
    At the moment she is looking for secondary antibody anty FC fragment of humanIgA,IgG and IgM (goat would be the best).

    Thank you for your enquiry.

    ab65623 is a good choice for developing western blots which use HRP conjugated secondary antibodies.

    Did your customer want one antibody that would react with the Fc region of IgA, IgG and IgM or did they wa...

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    Dear Sir/Madam Please let me know the difference between Chemiluminescent HRP Substrate (membrane) (ab5801) and ECL Western Blotting Substrate Kit (ab65623) because the customers do not know which one to choose for WB. Best regards.

    Thank you for your enquiry. I can confirm that both products (ab5801 and ab65623) can be used for Western blot application, however ab65623 can be applied for more test (50). Your customer may be interested in our very new range of WB reagents (Optibl...

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    Dear Technical Team, Hope this e-mail finds you well! Kindly arrange to send us the certificate of compliance (CoC) for the below Abcam products as desired by one of our customers : Cat No. Description ab97021 Goat polyclonal Secondary A...

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    I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"