ECL Western Blotting Substrate Kit - 50 Tests (ab65623)


  • Product nameECL Western Blotting Substrate Kit - 50 Tests
    See all ECL kits
  • Product overview

    The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.


    Looking for our new high sensitivity Optiblot ECL kits. View them here.

  • Notes

    Reagents provided are sufficient for 400 cm2 of membrane.

  • Tested applicationsSuitable for: WBmore details


  • RelevanceECL (enhanced luminol-based chemiluminescent) detection is a sensitive, nonradioactive method for easy detection of horseradish peroxidase (HRP) on western blots.
  • Alternative names
    • Enhanced luminol based chemiluminescent


Our Abpromise guarantee covers the use of ab65623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

ECL Western Blotting Substrate Kit - 50 Tests images

  • ECL Western Blotting Substrate

References for ECL Western Blotting Substrate Kit - 50 Tests (ab65623)

This product has been referenced in:
  • Leeming DJ  et al. Enzyme-linked immunosorbent serum assays (ELISAs) for rat and human N-terminal pro-peptide of collagen type I (PINP)--assessment of corresponding epitopes. Clin Biochem 43:1249-56 (2010). Read more (PubMed: 20709044) »
  • Mohan S  et al. Typhonium flagelliforme induces apoptosis in CEMss cells via activation of caspase-9, PARP cleavage and cytochrome c release: its activation coupled with G0/G1 phase cell cycle arrest. J Ethnopharmacol 131:592-600 (2010). WB . Read more (PubMed: 20673794) »

See all 2 Publications for this product

Product Wall

We have not specifically tested this Anti-CD9 antibody [EPR2949] (ab92726) as a marker of urinary exosomes but I can confirm that it can be used in western blot on mouse, rat, and human samples. Additionally, I have done a Google search and found that ...

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I am sorry do you need protcol or datasheet? The datasheet is attached.

I hope this information is helpful to you. Ple...

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Thank you for your enquiry.

ab65623 is a good choice for developing western blots which use HRP conjugated secondary antibodies.

Did your customer want one antibody that would react with the Fc region of IgA, IgG and IgM or did they...

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Thank you for your enquiry. I can confirm that both products (ab5801 and ab65623) can be used for Western blot application, however ab65623 can be applied for more test (50). Your customer may be interested in our very new range of WB reagents (Opt...

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I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.