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ECL Western Blotting Substrate Kit - 500 Tests (ab65628)

Q&A (1)

Overview

  • Product nameECL Western Blotting Substrate Kit - 500 TestsSee all ECL kits ...
  • Product overview

    The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. This ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.

     

    Looking for our new high sensitivity Optiblot ECL kits. View them here.

  • Tested applicationsWB more details

Properties

  • RelevanceECL (enhanced luminol-based chemiluminescent) detection is a sensitive, nonradioactive method for easy detection of horseradish peroxidase (HRP) on western blots.
    • Alternative names
        Enhanced luminol based chemiluminescent

    Applications

    Our Abpromise guarantee covers the use of ab65628 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB

    References for ECL Western Blotting Substrate Kit - 500 Tests (ab65628)

    ab65628 has not yet been referenced specifically in any publications.

    Publishing research using ab65628 ? Please let us know so that we can cite the reference in this datasheet

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    Customer said the secondary ab6721is working fine with other Abcam antibodies they bought in the same order, all from rabbit:

    - Beta actin = ab8227
    - Synaptophysin = ab52636
    - OPA1 = ab101589

    Thank you for your reply.

    I am sorry that the customer has had problems with these antibodies. As we guarantee thatboth ab2000 and ab32351 to work in western blot on rat tissue and they are not working as we expect then I am happy to either repl...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"