Overview

  • Product nameAnti-ECSIT antibodySee all ECSIT primary antibodies ...
  • Description
    Rabbit polyclonal to ECSIT
  • Tested applicationsICC/IF, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to 14 amino acids near the C-terminus of ECSIT (Human).

  • Positive control
    • human heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab21288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 21163940
IP Use at an assay dependent dilution. PubMed: 21163940
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 49 kDa).

Target

  • FunctionAdapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity). Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.
  • Sequence similaritiesBelongs to the ECSIT family.
  • Cellular localizationCytoplasm. Nucleus. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ecsit antibody
    • ECSIT homolog antibody
    • ECSIT protein antibody
    • ECSIT_HUMAN antibody
    • Evolutionarily conserved signaling intermediate in Toll pathway antibody
    • Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial antibody
    • Evolutionarily conserved signaling intermediate in Toll pathways antibody
    • Likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved antibody
    • Protein SITPEC antibody
    • Signaling intermediate in Toll pathway evolutionarily conserved antibody
    • SITPEC antibody
    • SITPEC protein antibody
    see all

Anti-ECSIT antibody images

  • Lane 1 : Anti-ECSIT antibody (ab21288) at 0.5 µg/ml
    Lane 2 : Anti-ECSIT antibody (ab21288) at 1 µg/ml
    Lane 3 : Anti-ECSIT antibody (ab21288) at 2 µg/ml

    Lane 1 : human heart lysate
    Lane 2 : human heart lysate
    Lane 3 : human heart lysate


    Predicted band size : 49 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • ab21288 at 2µg/ml staining ECSIT in mouse heart by IHC

References for Anti-ECSIT antibody (ab21288)

This product has been referenced in:
  • Guarani V  et al. TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Mol Cell Biol 34:847-61 (2014). Read more (PubMed: 24344204) »
  • Soler-López M  et al. Interactome mapping suggests new mechanistic details underlying Alzheimer's disease. Genome Res 21:364-76 (2011). WB, ICC/IF, IP ; Human . Read more (PubMed: 21163940) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry and your interest.

I regret to inform you that the immunogen sequence is commercially sensitive information and we are not allowed to release it. However, it is between residues 301 and 350 aa accession number NP_05...

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Thank you very much for your reply and Nezira's. At this stage I think it would be worth looking at Nezira's protocol in detail in light of the fact that the first lot did not give her the results expected. Can you please ask Nezira to click on th...

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Thank you again for your enquiry. I spoke to a colleague of mine who also agreed that you may be detecting the endogenous protein. Did you cleave the tags? The tags may be adding the additional kDa’s to your bands. Another possibility is that differ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"