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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Overview
Product name
Anti-ECSIT antibody
See all ECSIT products (2) ...
Description
Rabbit polyclonal to ECSIT
Tested applications
ICC/IF, IP, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to 14 amino acids near the C-terminus of ECSIT (Human).
Positive control
human heart lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purification notes
Ion exchange chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (PubMed: 21163940)
IP: Use at an assay dependent dilution. (PubMed: 21163940)
IHC-P: Use a concentration of 2 µg/ml
WB: Use a concentration of 0.5 - 1 µg/ml.Detects a band of approximately 42 kDa (predicted molecular weight: 49 kDa).
Target
Function
Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity). Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.
Sequence similarities
Belongs to the ECSIT family.
Cellular localization
Cytoplasm. Nucleus. Mitochondrion.
Target information above from: UniProt accessionQ9BQ95
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ecsit antibody
- ECSIT homolog antibody
- ECSIT protein antibody
see all
Database links
- Entrez Gene: 51295 Human
- Entrez Gene: 26940 Mouse
- Omim: 608388 Human
- SwissProt: Q96HQ7 Human
- SwissProt: Q9BQ95 Human
- SwissProt: Q9NYI1 Human
- SwissProt: Q91V53 Mouse
- SwissProt: Q9QZH6 Mouse
see all
Anti-ECSIT antibody images:
Western blot - ECSIT antibody (ab21288)
Lane 1 : Anti-ECSIT antibody (ab21288) at 0.5 µg/ml
Lane 2 : Anti-ECSIT antibody (ab21288) at 1 µg/ml
Lane 3 : Anti-ECSIT antibody (ab21288) at 2 µg/ml
Lane 1 : human heart lysate
Lane 2 : human heart lysate
Lane 3 : human heart lysate
Predicted band size : 49 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ECSIT antibody (ab21288)
ab21288 at 2µg/ml staining ECSIT in mouse heart by IHC
References for Anti-ECSIT antibody (ab21288)
This product has been referenced in:
- Soler-López Met al. Interactome mapping suggests new mechanistic details underlying Alzheimer's disease. Genome Res 21:364-76 (2011). WB, ICC/IF, IP; Human.Read more (PubMed: 21163940) »
- Vogel ROet al. Cytosolic signaling protein Ecsit also localizes to mitochondria where it interacts with chaperone NDUFAF1 and functions in complex I assembly. Genes Dev 21:615-24 (2007).Read more (PubMed: 17344420) »
See all 2 publications for this product
Publishing research using ab21288? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"