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Product Name
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ECSIT peptide
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Product type
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Proteins and Peptides
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Peptide
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Synthetic peptide corresponding to 14 amino acids near the C-terminus of ECSIT (Human).
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Tested applications
(see key)
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BL, WB
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Application notes
(see key)
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Recommended dilutions
BL: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
ECSIT peptide is used for blocking the activity of ECSIT antibody ab21288. It blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
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Relevance
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ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) is an intermediate in the Toll/IL1 signal transduction pathway. Activation of NFkB as a result of Toll-like receptor (TLR) and IL1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)6 in the TLR (Toll-like receptor) pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/ILB and Bmp signaling pathways.
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ECSIT peptide is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to ECSIT
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ECSIT peptide - more information
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Customer reviews (feedback) regarding ECSIT peptide |
Customer FAQs regarding ECSIT peptide |
Protocols for ECSIT peptide |
Price and availability of products related to ECSIT peptide |
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ECSIT peptide - related products:
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ECSIT peptide - other tools:
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Contact Abcam with a Technical Enquiry about ECSIT peptide |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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