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Anti-ECT2 antibody (ab4863)

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Overview

Product name

Anti-ECT2 antibody
See all ECT2 products (4) ...

Description

Goat polyclonal to ECT2

Tested applications

WB, ICC/IFmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide: AENSVLTSTTGRT, corresponding to N terminal amino acids 2-14 of Human ECT2.

AENSVLTSTT GRT-C

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

General notes

Gene Ontology terms - guanyl-nucleotide exchange factor activity; cell growth and/or maintenance; intracellular signaling cascade; intracellular.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Cell Cycle >> Markers

  • Immunofluorescence - ECT2 antibody (ab4863)Immunofluorescence - ECT2 antibody (ab4863) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab4863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/500.
WB: 1/200 - 1/500 (overnight incubation in blocking buffer). Block with 5% milk for 30 min to 1 hr.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.

Cellular localization

ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis

Alternative names

  • ECT 2 antibody
  • ECT2 protein antibody
  • Epithelial Cell Transforming Sequence 2 antibody
  • Epithelial cell transforming sequence 2 oncogene protein antibody
  • FLJ10461 antibody
  • MGC138291 antibody
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Database links

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Anti-ECT2 antibody images:

  Immunofluorescence - ECT2 antibody (ab4863)

Immunofluorescence - ECT2 antibody (ab4863)

This image was kindly supplied as part of the review submitted by Naslin Rasheed.

MEF cells were incubated with ab4863 (1/500) for 2 hours at room temperature. The staining shows the protein is localised to the nucleus.

References for Anti-ECT2 antibody (ab4863)

This product has been referenced in:

See 1 publication for this product

Publishing research using ab4863? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"