Overview

  • Product name
  • Description
    Goat polyclonal to ECT2
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide: AENSVLTSTTGRT, corresponding to N terminal amino acids 2-14 of Human ECT2.

  • General notes
    Gene Ontology terms - guanyl-nucleotide exchange factor activity; cell growth and/or maintenance; intracellular signaling cascade; intracellular.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/500. Can be blocked with Human ECT2 peptide (ab23060).

Overnight incubation in blocking buffer. Block with 5% milk for 30 min to 1 hr.

ICC/IF 1/500.

Target

  • Relevance
    The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
  • Cellular localization
    ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis
  • Database links
  • Alternative names
    • ECT 2 antibody
    • ECT2 protein antibody
    • Epithelial cell transforming sequence 2 antibody
    • Epithelial cell transforming sequence 2 oncogene antibody
    • Epithelial cell transforming sequence 2 oncogene protein antibody
    • FLJ10461 antibody
    • MGC138291 antibody
    see all

Images

  • This image was kindly supplied as part of the review submitted by Naslin Rasheed.

    MEF cells were incubated with ab4863 (1/500) for 2 hours at room temperature. The staining shows the protein is localised to the nucleus.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Specification
colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M Tris Buffer (pH9.0)/ 0.01M citrate Buffer (pH6.0)
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Ms. JIHYE HAN

Verified customer

Submitted Jan 29 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Jul 23 2009

ab4863 is an investigative grade antibody which means it has only been characterised by ELISA against the immunogen. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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