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Immunofluorescence - ECT2 antibody (ab4863)
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Product Name
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ECT2 antibody
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See all ECT2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to ECT2
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Immunogen
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Synthetic peptide: AENSVLTSTTGRT, corresponding to N terminal amino acids 2-14 of Human ECT2.
BLAST 'AENSVLTSTTGRT-C' with  or 
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Reacts with
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Hu
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Predicted to react
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Ms
(Due to sequence homology)
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/500.
WB: 1/200 - 1/500 (overnight incubation in blocking buffer). Block with 5% milk for 30 min to 1 hr.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis
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Research areas
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Cell Biology >> Cell Cycle >> Markers
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Relevance
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The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Gene Ontology terms - guanyl-nucleotide exchange factor activity; cell growth and/or maintenance; intracellular signaling cascade; intracellular.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ECT2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ECT2 antibody images, references, products related to ab4863 and other tools.
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ECT2 antibody images:
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 Immunofluorescence - ECT2 antibody (ab4863)
This image was kindly supplied as part of the review submitted by Naslin Rasheed.
MEF cells were incubated with ab4863 (1/500) for 2 hours at room temperature. The staining shows the protein is localised to the nucleus.
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Search PubMed (MEDLINE) for references to ECT2
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ECT2 antibody - more information
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Customer reviews (feedback) regarding ECT2 antibody |
Customer FAQs regarding ECT2 antibody |
Protocols for ECT2 antibody |
Price and availability of products related to ECT2 antibody |
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ECT2 antibody - related products:
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ECT2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ECT2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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