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Anti-ECT2 (phospho T790) antibody (ab51494)

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Overview

Product name

Anti-ECT2 (phospho T790) antibody
See all ECT2 products (4) ...

Description

Rabbit polyclonal to ECT2 (phospho T790)

Specificity

Reactivity occurs against human ECT2 protein at the pT790 residue.

Tested applications

WB, ELISA, ICC/IFmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Dog, Chimpanzee, Zebrafish

Immunogen

Synthetic peptide: TKDMDSTLSRA, corresponding to amino acids 785-795 of Human ECT2

TKDMDSTLSR A

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HeLa cell lysates

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab51494 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Cell Cycle >> Markers

Applications

Show applications key

Our Abpromise guarantee covers the use of ab51494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: 1/10000 - 1/40000.
ICC/IF: Use at an assay dependent dilution.
WB: 1/1000 - 1/4000. Predicted molecular weight: 100 kDa.
Less than 2% reactivity is observed against the non-phosphorylated form of
the immunizing peptide.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.

Cellular localization

ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis

Database links

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References for Anti-ECT2 (phospho T790) antibody (ab51494)

ab51494 has not yet been referenced specifically in any publications.

Publishing research using ab51494? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"