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Western blot - EDA antibody (ab84311)
(enlarge)
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Product Name
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EDA antibody
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See all EDA antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EDA
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Immunogen
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Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (HLQGQGSAIQ VKNDLSGGVL NDWSRITMNP KVFKLHPRSG ELEVLVDGTY) of Human EDA (NP_001005609).
BLAST 'HLQGQGSAIQ VKNDLSGGVL NDWSRITMNP KVFKLHPRSG ELEVLVDGTY ' with  or 
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate.
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Cellular localization
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Cell membrane; Single-pass type II membrane protein. Secreted.
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Research areas
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Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Lineage Markers >> Ectoderm
Signal Transduction >> Growth Factors/Hormones >> TNF
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Relevance
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The protein encoded by the EDA gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in the EDA gene are a cause of ectodermal dysplasia, anhidrotic, (this is also known as X-linked hypohidrotic ectodermal dysplasia). Several transcript variants encoding many different isoforms have been found for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EDA antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EDA antibody images, references, products related to ab84311 and other tools.
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EDA antibody images:
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 Western blot - EDA antibody (ab84311)
EDA antibody (ab84311) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 42 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
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Search PubMed (MEDLINE) for references to EDA
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EDA antibody - more information
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Customer reviews (feedback) regarding EDA antibody |
Customer FAQs regarding EDA antibody |
Protocols for EDA antibody |
Price and availability of products related to EDA antibody |
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EDA antibody - related products:
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EDA antibody - other tools:
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Contact Abcam with a Technical Enquiry about EDA antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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