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Products:Signal Transduction >> Growth Factors/Hormones >> TNF
Overview
Product name
Anti-EDA antibody
See all EDA products (3) ...
Description
Rabbit polyclonal to EDA
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Chicken, Cow, Dog
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (HLQGQGSAIQ VKNDLSGGVL NDWSRITMNP KVFKLHPRSG ELEVLVDGTY) of Human EDA (NP_001005609).
HLQGQGSAIQ VKNDLSGGVL NDWSRITMNP KVFKLHPRSG ELEVLVDGTY
Positive control
HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Lineage Markers >> Ectoderm
Signal Transduction >> Growth Factors/Hormones >> TNF
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Western blot - EDA antibody (ab84311)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 42 kDa.
Target
Function
Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR.
Tissue specificity
Not abundant; expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord.
Involvement in disease
Defects in EDA are the cause of ectodermal dysplasia type 1 (ED1) [MIM:305100]; also known as Christ-Siemens-Touraine syndrome or X-linked hypohidrotic ectodermal dysplasia (XLHED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED1 is a disease characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. ED1 is the most common form of over 150 clinically distinct ectodermal dysplasias.
Defects in EDA are the cause of tooth agenesis selective X-linked type 1 (STHAGX1) [MIM:313500]. A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth).
Sequence similarities
Belongs to the tumor necrosis factor family.
Contains 1 collagen-like domain.
Post-translational
modifications
N-glycosylated.
Processing by furin produces a secreted form.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ92838
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- secreted form antibody
- Ectodermal dysplasia 1, anhidrotic antibody
- Ectodermal dysplasia protein antibody
see all
Database links
- Entrez Gene: 1896 Human
- Entrez Gene: 13607 Mouse
- Omim: 300451 Human
- SwissProt: Q9BEG5 Cow
- SwissProt: Q92838 Human
- SwissProt: O54693 Mouse
- Unigene: 105407 Human
- Unigene: 328086 Mouse
Anti-EDA antibody images:
Western blot - EDA antibody (ab84311)
Anti-EDA antibody (ab84311) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 42 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
References for Anti-EDA antibody (ab84311)
ab84311 has not yet been referenced specifically in any publications.
Publishing research using ab84311? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"