You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-EDC4 antibody (ab72408)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-EDC4 antibody
See all EDC4 products (3) ...

Description

Rabbit polyclonal to EDC4

Tested applications

WB, IP, ICC/IF, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic peptide from a region between residue 1 and 50 of human EDC4, AAH43616.

Positive control

Whole cell lysate from Hela, 293T and NIH3T3 cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - EDC4 antibody (ab72408)Western blot - EDC4 antibody (ab72408) image (enlarge)

  • Immunoprecipitation - EDC4 antibody (ab72408)Immunoprecipitation - EDC4 antibody (ab72408) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence-EDC4 antibody(ab72408)Immunocytochemistry/ Immunofluorescence-EDC4 antibody(ab72408) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab72408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).

Sequence similarities

Belongs to the WD repeat EDC4 family.
Contains 4 WD repeats.

Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.

Cellular localization

Cytoplasm > P-body. Nucleus.

Target information above from: UniProt accessionQ6P2E9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • autoantigen antibody
  • Autoantigen Ge 1 antibody
  • Autoantigen Ge-1 antibody
  • Autoantigen Ge1 antibody
  • Autoantigen RCD 8 antibody
  • Autoantigen RCD-8 antibody
  • Autoantigen RCD8 antibody
  • edc4 antibody
  • EDC4_HUMAN antibody
  • Enhancer of mRNA decapping 4 antibody
  • Enhancer of mRNA-decapping protein 4 antibody
  • Ge 1 antibody
  • Hedls antibody
  • Human enhancer of decapping large subunit antibody
  • RCD 8 antibody
see all

Anti-EDC4 antibody images:

  Western blot - EDC4 antibody (ab72408)

Western blot - EDC4 antibody (ab72408)

All lanes : Anti-EDC4 antibody (ab72408) at 0.04 µg/ml

Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

developed using the ECL technique

Predicted band size : 152 kDa
Observed band size : 190 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 30 seconds

  Immunoprecipitation - EDC4 antibody (ab72408)

Immunoprecipitation - EDC4 antibody (ab72408)

Immunoprecipitation/ Western Blot of EDC4.
Lane 1: ab72408 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.

ab72408 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.

  Immunocytochemistry/ Immunofluorescence-EDC4 antibody(ab72408)

Immunocytochemistry/ Immunofluorescence-EDC4 antibody(ab72408)

ICC/IF image of ab72408 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72408, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EDC4 antibody (ab72408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EDC4 antibody (ab72408)

IHC image of ab72408 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72408, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-EDC4 antibody (ab72408)

This product has been referenced in:

  • Chang WL & Tarn WY A role for transportin in deposition of TTP to cytoplasmic RNA granules and mRNA decay. Nucleic Acids Res 37:6600-12 (2009). ICC/IF; Human.Read more (PubMed: 19729507) »

See 1 publication for this product

Publishing research using ab72408? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"