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Western blot - EDC4 antibody (ab72408)
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Immunoprecipitation - EDC4 antibody (ab72408)
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Product Name
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EDC4 antibody
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See all EDC4 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EDC4
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Immunogen
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Synthetic peptide from a region between residue 1 and 50 of human EDC4, AAH43616.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution (PMID 19729507).
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 55 and 190 kDa (predicted molecular weight: 152 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole cell lysate from Hela, 293T and NIH3T3 cells.
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Cellular localization
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Cytoplasm, P-body. Nucleus.
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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EDC4 belongs to the WD repeat EDC4 family, it contains four WD repeats. In the process of mRNA degradation EDC4 seems to play a role in mRNA decapping. EDC4 is a component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. It promotes complex formation between DCP1A and DCP2, and enhances the catalytic activity of DCP2 (in vitro). There are two named isoforms.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EDC4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EDC4 antibody images, references, products related to ab72408 and other tools.
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EDC4 antibody images:
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 Western blot - EDC4 antibody (ab72408)
All lanes : EDC4 antibody (ab72408) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
developed using the ECL technique
Predicted band size : 152 kDa
Observed band size : 190 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
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Immunoprecipitation - EDC4 antibody (ab72408)
Immunoprecipitation/ Western Blot of EDC4.
Lane 1: ab72408 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72408 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
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References for EDC4 antibody (ab72408)
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This product has been used in:
Chang WL & Tarn WY A role for transportin in deposition of TTP to cytoplasmic RNA granules and mRNA decay. Nucleic Acids Res 37:6600-12 (2009). ICC/IF; Human. PubMed: 19729507
If you publish research using ab72408 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to EDC4
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EDC4 antibody - more information
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Customer reviews (feedback) regarding EDC4 antibody |
Customer FAQs regarding EDC4 antibody |
Protocols for EDC4 antibody |
Price and availability of products related to EDC4 antibody |
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EDC4 antibody - related products:
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EDC4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about EDC4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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