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Product name
Anti-EDD antibody
See all EDD products (6) ...
Description
Goat polyclonal to EDD
Tested applications
WB, IP, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rabbit, Human
Predicted to work with
Rat, Cow
Immunogen
Synthetic peptide: LAIKTKNFGFV, corresponding to C terminal amino acids 2789-2799 of Human EDD.
C-LAIKTKNFGFV
General notes
GenBank Accession Number – NP_056986.
LocusLink ID - 51366 (human); 70790 (mouse); 117060 (rat).
Gene Ontology terms - ubiquitin conjugating enzyme activity; ubiquitin-protein ligase activity; ubiquitin cycle; cell proliferation; protein modification; soluble fraction; nucleus; ligase activity.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).
IP: Use at an assay dependent dilution.
IHC-P: Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.No results could not be obtained after antigen retrieval at pH6.
ICC/IF: Use at an assay dependent concentration. PubMed: 21148289
Target
Function
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
Tissue specificity
Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 PABC domain.
Contains 1 UBR-type zinc finger.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO95071
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AW549941 antibody
- C77315 antibody
- D030042K14 antibody
see all
Database links
- Entrez Gene: 515582 Cow
- Entrez Gene: 51366 Human
- Entrez Gene: 70790 Mouse
- Entrez Gene: 117060 Rat
- Omim: 608413 Human
- SwissProt: O95071 Human
- SwissProt: Q80TP3 Mouse
- SwissProt: Q62671 Rat
see all
Anti-EDD antibody images:
Western blot - EDD antibody (ab4376)
Anti-EDD antibody (ab4376) at 1/500 dilution + MDA-MB-468 breast cancer cells (25µg)
Secondary
Donkey anti-goat conjugated to HRP at 1/4000 dilution
Performed under reducing conditions.
Predicted band size : 309 kDa
Exposure time : 5 seconds
Developed using SuperBlock Blocking Buffer (pierce) and pico ECL kit (pierce) to reduce the backgound observed - please refer to abreview for further experimental details.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab4376)
ab4376 (4µg/ml) staining of paraffin embedded Human Testis shows nuclear staining . Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.
References for Anti-EDD antibody (ab4376)
This product has been referenced in:
- Hay-Koren A et al. The EDD E3 ubiquitin ligase ubiquitinates and up-regulates beta-catenin. Mol Biol Cell 22:399-411 (2011). WB; Human.Read more (PubMed: 21118991) »
- Won JH et al. Phenotypic changes in mouse pancreatic stellate cell Ca2+ signaling events following activation in culture and in a disease model of pancreatitis. Mol Biol Cell 22:421-36 (2011). ICC/IF.Read more (PubMed: 21148289) »
See all 2 publications for this product
Publishing research using ab4376? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"