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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
Overview
Product name
Anti-EDF1 antibody
See all EDF1 products (4) ...
Description
Rabbit polyclonal to EDF1
Specificity
ab33588 recognises endothelial differentiation-related factor 1 (EDF1).
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Pig
Immunogen
Synthetic peptide: MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK, corresponding to a region within N terminal amino acids 1-50 of Human EDF1.
MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK
Positive control
Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - EDF1 antibody (ab33588)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlPredicted molecular weight: 16 kDa.
ELISA
ELISA: 1/312500ELISA: 1/312500
Target
Function
Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
Tissue specificity
Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues.
Sequence similarities
Contains 1 HTH cro/C1-type DNA-binding domain.
Developmental stage
Expressed in fetal tissues. More abundant in kidney.
Domain
The IQ motif, which is involved in calmodulin binding, overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein.
Post-translational
modifications
Phosphorylated (by PKA and PKC).
Cellular localization
Cytoplasm. Nucleus. Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin.
Target information above from: UniProt accessionO60869
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAP19 antibody
- EDF-1 antibody
- edf1 antibody
see all
Database links
- Entrez Gene: 417286 Chicken
- Entrez Gene: 515380 Cow
- Entrez Gene: 8721 Human
- Entrez Gene: 59022 Mouse
- Entrez Gene: 296570 Rat
- Omim: 605107 Human
- SwissProt: Q3T0V7 Cow
- SwissProt: O60869 Human
see all
Anti-EDF1 antibody images:
Western blot - EDF1 antibody (ab33588)
Anti-EDF1 antibody (ab33588) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti Rabbit IgG at 1/50000 - 1/100000
Predicted band size : 16 kDa
Observed band size : 16 kDa
References for Anti-EDF1 antibody (ab33588)
ab33588 has not yet been referenced specifically in any publications.
Publishing research using ab33588? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"