Anti-EDG1 antibody (ab11424)

I would like to test ab11424 with rat samples in western blotting.

ANSWER:

I am very pleased to hear you would like to accept our offer and test ab11424 in rat. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forrat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

This antibody is showing bands in both my WT and KO mouse model.

ANSWER:

Thank you for contacting us and I am sorry to hear that ab11424 is not providing satisfactory results.

I am going to refund this purchase for you. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

I purchased ab11424 a Rabbit Polyclonal to EDG1, and used it in WB and mouse which seems to be guaranteed on the datasheet. I had very weak bands and had better results with a similar ab from another company.

ANSWER:

Thank you for making us aware that you experienced a problem with our EDG1 antibody ab11424.

As my colleague mentioned, this product is guaranteed to work with mouse samples in WB and can be replaced or refunded per our Abpromise.  For our records, I would like to gather some additional information about the protocol you tested and your samples.  Can you please provide a protocol summary and an image of your results?  We will use this information to investigate this problem further. Should we determine that the antibody is not working as stated on the datasheet, I would be happy to offer a replacement or credit.  Please let me know which you would prefer.

I look forward to your reply so that I may assist you further.  Please do not hesitate to contact us if you have any additonal questions.

How were the cells fixed for ICC/IF using this antibody? I would also like to see an image.

ANSWER:

Thank you for your inquiry.

I heard back from the testing laboratory regarding the fixation/permeabilization protocol for ab11424.

Aspirate culture medium and add warmed Fixation Solution (4% Formaldehyde, 37C) and incubate 15 minutes at room temperature. Aspirate Fixation Solution and wash with Dulbecco’s PBS. Aspirate solution. Add Permeabilization Solution (Dulbecco’s PBS with 0.1% Trition X100) and incubate 15 minutes at room temperature. Aspirate solution and proceed to blocking/antibody steps.

I hope this information helps. I hope to have the ICC/IF image on the datasheet shortly. Please contact us with any other questions.

This enquiry is in regard to EDG1 antibodies (both ab11424 and ab13126). Ultimately we would like to use an EDG1 antibody to stain formalin-fixed paraffin embedded tissue sections of rhesus macaques (monkeys).

The product sheet for ab11424 states that the antibody can be used for immunofluorescence, but does this include immunofluorescent detection on tissue sections (fixed, frozen or paraffin embedded)?

Ab13126 appears to work in formalin-fixed paraffin-embedded sections, but has not been tested in any other species except humans, is this antibody expected to cross-react with a broad range of species like the ab11424 antibody?

Also, could either of these antibodies be used to stain unfixed cells for flow cytometric analysis?

ANSWER:

Thank you for your email. When ab11424 was tested in IF, transfected CCL39 cells were used. So to our knowledge it has not yet been tested in IHC. Ab13126 has only been tested for cross-reactivity with human and we don't have any information about how it will work with other species. Neither antibody has been tested yet for application in Flow Cytometry. If you have any more questions, please contact us again.