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Products:Cell Biology >> Apoptosis >> Receptors >> Associated Proteins
Overview
Product name
Anti-EDG1 antibody
See all EDG1 products (8) ...
Description
Rabbit polyclonal to EDG1
Specificity
Ab11424 detects S1P1 protein (EDG1) in transfected human cell samples. This antibody shows no cross-reactivity to S1P3.
Tested applications
IF, WB, IP, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Horse, Cat, Monkey
Immunogen
Synthetic peptide: SHPQKDDGDNPETI, corresponding to amino acids 359-372 of Mouse EDG1 (Peptide available as ab39763.)
SHPQKDDGDN PETI
Positive control
Transfected HEK293 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1mg/ml BSA, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial
Cell Biology >> Apoptosis >> Receptors >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use a concentration of 5 µg/ml(Immunofluorescence data demonstrates that S1P1 is localized to the plasma membrane after staining with ab11424.)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 44 kDa.(Band represents recombinant S1P1 from transfected HEK293 cells.)
IP: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution. (PubMed: 20733200)
Target
Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
Tissue specificity
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Post-translational
modifications
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP21453
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD363 antibody
- CHEDG 1 antibody
- CHEDG1 antibody
see all
Database links
- Entrez Gene: 1901 Human
- Entrez Gene: 13609 Mouse
- Entrez Gene: 29733 Rat
- Omim: 601974 Human
- SwissProt: P21453 Human
- SwissProt: O08530 Mouse
- SwissProt: P48303 Rat
- Unigene: 154210 Human
see all
Anti-EDG1 antibody images:
Immunocytochemistry/ Immunofluorescence - EDG1 antibody (ab11424)
ab11424 staining EDG1 in transfected CCL39 cells by Immunocytochemistry/ Immunofluorescence.
Western blot - EDG1 antibody (ab11424)
ab11424 staining EDG1 in Western Blot using a transfected HEK293 cell lysate.
References for Anti-EDG1 antibody (ab11424)
This product has been referenced in:
- Illuzzi Get al. Sphingosine kinase mediates resistance to the synthetic retinoid N-(4-hydroxyphenyl)retinamide in human ovarian cancer cells. J Biol Chem 285:18594-602 (2010). WB; Human.Read more (PubMed: 20404323) »
- Nincheri Pet al. Sphingosine kinase-1/S1P1 signalling axis negatively regulates mitogenic response elicited by PDGF in mouse myoblasts. Cell Signal 22:1688-99 (2010). WB; Mouse.Read more (PubMed: 20600848) »
See all 4 publications for this product
Publishing research using ab11424? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"