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Products:Cell Biology >> Apoptosis >> Receptors >> Associated Proteins
Overview
Product name
Anti-EDG1 antibody
See all EDG1 products (8) ...
Description
Rabbit polyclonal to EDG1
Specificity
The S1P1 antibody is directed against a cytoplasmic loop of this receptor so a punctuate staining is to be expected (see image). This result is similar to the result we have had with other antibodies that are directed to the intracellular side of receptor proteins. Since the antibody is on the intracellular side, accumulation of the colorimetric signal can occur there and give the effect of staining the cytoplasm. The liver staining observed looks to be associated with intracellular bodies, with some staining at the ER/nuclear interfaces. One interpretation is that the protein is associated with membranes, but for some reason not restricted specifically with the plasma membrane. There is little known, or published about this protein currently.
Tested applications
WB, IHC-P, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig
Immunogen
Synthetic peptide: ISKASRSSEKSLA, corresponding to amino acids 241-253 of Human EDG1
ISKASRSSEK SLA
Positive control
Human and pig liver, mouse and rat brain.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, Tris buffered saline, pH 7.4
Concentration
Concentration information loading...
Purification notes
ab23695 is an affinity purified IgG.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial
Cell Biology >> Apoptosis >> Receptors >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/mlPredicted molecular weight: 42 kDa.
IHC-P: Use a concentration of 4 µg/ml
ICC: Use a concentration of 8 µg/ml(Wash cells in TBS pH 7.4. Fix cells in 1% paraformaldehyde in TBS for 10 min. Wash cells in TBST (1% Tritonx100) 3 times. Block in normal serum 10% for 30 min. Remove the serum and add the primary antibody. Incubate for 1hr. Wash cells and incubate with fluorescent conjugated secondary antibody)
Target
Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
Tissue specificity
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Post-translational
modifications
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP21453
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD363 antibody
- CHEDG 1 antibody
- CHEDG1 antibody
see all
Database links
- Entrez Gene: 1901 Human
- Entrez Gene: 13609 Mouse
- Entrez Gene: 29733 Rat
- Omim: 601974 Human
- SwissProt: P21453 Human
- SwissProt: O08530 Mouse
- SwissProt: P48303 Rat
- Unigene: 154210 Human
see all
Anti-EDG1 antibody images:
Western blot - EDG1 antibody (ab23695)
All lanes : Anti-EDG1 antibody (ab23695) at 2 µg/ml
Lane 1 : 30ug of human liver microsomes.
Lane 2 : 30ug mouse brain homogenate.
Lane 3 : 30ug pig liver 10000 x g supernatant
Lane 4 : 30ug rat brain homogenate.
Predicted band size : 42 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - EDG1 antibody (ab23695)
Immunohistochemistry performed on formalin fixed, paraffin embedded sections of neuro2-A cells with ab23695 (8ug/ml). The secondary antibody was a cy3 conjugated donkey anti rabbit antibody.
References for Anti-EDG1 antibody (ab23695)
This product has been referenced in:
- Zhu Det al. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor. Blood 115:4963-72 (2010). WB; Human.Read more (PubMed: 20348395) »
- Jang Cet al. Angiopoietin-2 exocytosis is stimulated by sphingosine-1-phosphate in human blood and lymphatic endothelial cells. Arterioscler Thromb Vasc Biol 29:401-7 (2009).Read more (PubMed: 19112163) »
See all 2 publications for this product
Publishing research using ab23695? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"