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ISKASRSSEKSLA, corresponding to amino acids 241-253 of Human EDG1
Our Abpromise guarantee covers the use of ab23695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 42 kDa.|
|IHC-P||Use a concentration of 4 µg/ml.|
|ICC||Use a concentration of 8 µg/ml. Wash cells in TBS pH 7.4. Fix cells in 1% paraformaldehyde in TBS for 10 min. Wash cells in TBST (1% Tritonx100) 3 times. Block in normal serum 10% for 30 min. Remove the serum and add the primary antibody. Incubate for 1hr. Wash cells and incubate with fluorescent conjugated secondary antibody|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"