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I have successfully used the abcam anti-EDG2 rabbit polyclonal antibody (ab23698) in mouse and human tissue sections. I am wondering if this antibody is very specific for EDG2 (LPAR1) or if it is possible that it may also detect EDG4 (LPAR2) in my tissue samples. |
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ANSWER: |
The antibody is specific for EDG2(LPAR1). We do not find any significant similarity of the peptide antigen used for LPAR1 antibody production to residues within the LPAR2 protein. The antibody should not detect EDG4 (LPAR2). I hope this is helpful. Please feel free to contact me with any further technical questions. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-EDG2 antibody (ab23698) at 2 µg/ml
Lane 1 : Mouse liver at 50 µg
Lane 2 : A549 cell lysate at 40 µg
Lane 3 : Rat liver lysate at 40 µg
Predicted band size : 41 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
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