EDG2 antibody (ab23698)

Product Name 

EDG2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to EDG2

Immunogen 

Synthetic peptide: DRSASSLNH TILAGVHSN, corresponding to amino acids 342-359 of Human EDG2.
BLAST 'DRSASSLNH TILAGVHSN' with or

Reacts with 

Hu, Ms, Rat, Cow, Shp

Predicted to react 
(species key)

Xl
(Due to sequence homology)

Tested applications 
(see key)

ICC, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: Use at a concentration of 8 µg/ml.
IHC-Fr: Use at an assay dependent dilution (PMID 18772233).
WB: Use at a concentration of 2 - 5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 41 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

For western blot, Mouse liver, A549 cell lysate, Rat Liver lysate.

Cellular localization 

Cell membrane; Multi-pass membrane protein.

Research areas 

Signal Transduction >> Metabolism >> Vitamins / Minerals
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC

Relevance 

EDG2 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. There are eight known members of the EDG receptor family and they are implicated in mediating growth-related effects such as induction of cellular proliferation, alterations in differentiation and survival, and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C, and several proteins not used within 12 hours. Edg2 has been reported in most human tissues, and is especially abundant in brain cortical regions. ESTs have been isolated from bone, brain, breast, connective tissue, embryo, heart/melanocyte/uterus, lung, prostate and uterus libraries.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 30% Glycerol, 0.1% BSA, TBS. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for EDG2 antibody images, references, products related to ab23698 and other tools.

EDG2 antibody images:

References for EDG2 antibody (ab23698)

  This product has been used in:

Liszewska E et al. Lysophosphatidic acid signaling during embryo development in sheep: involvement in prostaglandin synthesis. Endocrinology : (2008). WB, IHC-Fr; Sheep. PubMed: 18772233

If you publish research using ab23698 please let us know so that we can cite the reference on this datasheet.

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