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Anti-EDG2 antibody (ab23698)

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Overview

Product name

Anti-EDG2 antibody
See all EDG2 products (6) ...

Description

Rabbit polyclonal to EDG2

Tested applications

IHC-Fr, WB, ICCmore details

Cross reactivity

Reacts with

Mouse, Rat, Sheep, Cow, Human

Predicted to work with

Xenopus laevis

Immunogen

Synthetic peptide: DRSASSLNH TILAGVHSN, corresponding to amino acids 342-359 of Human EDG2.

DRSASSLNH TILAGVHSN

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

For western blot, Mouse liver, A549 cell lysate, Rat Liver lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 30% Glycerol, 0.1% BSA, Tris buffered saline, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Other
Signal Transduction >> Metabolism >> Vitamins / Minerals
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> PLC

  • Western blot - EDG2 antibody (ab23698)Western blot - EDG2 antibody (ab23698) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab23698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: Use at a concentration of 8 µg/ml.
IHC-Fr: Use at an assay dependent dilution (PMID 18772233).
WB: Use at a concentration of 2 - 5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 41 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.

Tissue specificity

Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Cellular localization

Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.

Target information above from: UniProt accessionQ92633 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 5031439C20 antibody
  • AI326300 antibody
  • EDG 2 antibody
  • Endothelial Cell Differentiation Gene-2 antibody
  • Endothelial differentiation gene 2 antibody
  • Endothelial Differentiation Lysophosphatidic Acid G Protein Coupled Receptor 2 antibody
  • Endothelial Differentiation Lysophosphatidic Acid G Protein Coupled Receptor 2 antibody
  • G10 protein homolog antibody
  • G10 protein homolog antibody
  • GPCR 26 antibody
  • GPCR26 antibody
  • GPCR26 antibody
  • Gpcr91 antibody
  • Kdt2 antibody
  • LPA 1 antibody
  • LPA receptor 1 antibody
  • LPA receptor 1 antibody
  • LPA receptor EDG2 antibody
  • LPA-1 antibody
  • LPA1 antibody
  • LPA1 antibody
  • Lpar1 antibody
  • LPAR1_HUMAN antibody
  • Lysophosphatidic acid receptor 1 antibody
  • Lysophosphatidic acid receptor Edg-2 antibody
  • Lysophosphatidic acid receptor EDG2 antibody
  • Lysophosphatidic acid receptor EDG2 antibody
  • MGC105279 antibody
  • MGC29102 antibody
  • MREC1.3 antibody
  • MREC1.3 antibody
  • rec.1.3 antibody
  • REC1.3 antibody
  • REC1.3 antibody
  • Ventricular zone gene 1 antibody
  • Ventricular zone gene 1 antibody
  • vzg-1 antibody
  • VZG1 antibody
  • VZG1 antibody
see all

Database links

see all

Anti-EDG2 antibody images:

  Western blot - EDG2 antibody (ab23698)

Western blot - EDG2 antibody (ab23698)

All lanes : Anti-EDG2 antibody (ab23698) at 2 µg/ml

Lane 1 : Mouse liver at 50 µg
Lane 2 : A549 cell lysate at 40 µg
Lane 3 : Rat liver lysate at 40 µg


Predicted band size : 41 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

References for Anti-EDG2 antibody (ab23698)

This product has been referenced in:

  • Zhang Het al. Proteomic analysis of bone tissues of patients with osteonecrosis of the femoral head. OMICS 13:453-66 (2009). WB; Human.Read more (PubMed: 20001860) »
  • Liszewska Eet al. Lysophosphatidic acid signaling during embryo development in sheep: involvement in prostaglandin synthesis. Endocrinology : (2008). WB, IHC-Fr; Sheep.Read more (PubMed: 18772233) »

See all 2 publications for this product

Publishing research using ab23698? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"