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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Overview
Product name
Anti-EDG3 antibody [AS61]
See all EDG3 products (5) ...
Description
Mouse monoclonal [AS61] to EDG3
Specificity
The antibody does not recognize EDG2, 4 or 5.
Tested applications
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic peptide to C-terminal region of EDG3 (Human).
Positive control
Human or rat spleen, or rat lung tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.035% Sodium Azide
Constituents: 30% Glycerol, 0.105M Sodium Chloride, 0.07M Tris-glycine. pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
The antibody is purified from ascites fluid using protein G chromatography.
Clonality
Monoclonal
Clone number
AS61
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Hypertension >> Vasoconstriction >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/10,000Detects a band of approximately 45 kDa (predicted molecular weight: 42.2 kDa).
Target
Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Tissue specificity
Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ99500
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EDG 3 antibody
- EDG3 antibody
- Endothelial differentiation G protein coupled receptor 3 antibody
see all
Database links
- Entrez Gene: 1903 Human
- Entrez Gene: 306792 Rat
- Omim: 601965 Human
- SwissProt: Q99500 Human
- Unigene: 585118 Human
References for Anti-EDG3 antibody [AS61] (ab12254)
ab12254 has not yet been referenced specifically in any publications.
Publishing research using ab12254? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"