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Western blot - EDG3 antibody (ab38324)
(enlarge)
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Product Name
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EDG3 antibody - Aminoterminal end
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See all EDG3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EDG3 - Aminoterminal end
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Immunogen
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Synthetic peptide: QPVRGNETLREHYQYVGKLAG conjugated to KLH, corresponding to N terminal amino acids 10/30 of Human EDG3
BLAST 'QPVRGNETLREHYQYVGKLAG' with  or 
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Reacts with
(species key)
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Hu, Ms
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Specificity
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This antibody is specific for EDG3 (N term).
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse kidney lysate.
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Cellular localization
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Integral Membrane Protein
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Research areas
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Cardiovascular >> Atherosclerosis >> Hypertension >> Vasoconstriction >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
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Relevance
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EDG3 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG3 is sphingosine-1-phosphate. There are eight known members of the EDG receptor family and they are implicated in mediating growth-related effects such as induction of cellular proliferation, alterations in differentiation and survival, and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C, and several protein tyrosine kinases. EDG3 is expressed in heart, placenta, kidney, and liver tissue.
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EDG3 antibody - Aminoterminal end (ab38324) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EDG3 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EDG3 antibody - Aminoterminal end images, references, products related to ab38324 and other tools.
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EDG3 antibody - Aminoterminal end images:
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 Western blot - EDG3 antibody (ab38324)
EDG3 antibody - Aminoterminal end (ab38324) at 1/100 dilution + mouse kidney lysate
Predicted band size : 42 kDa
Observed band size : 42 kDa
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to EDG3
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EDG3 antibody - Aminoterminal end - more information
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