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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Overview
Product name
Anti-EDG3 antibody - Aminoterminal end
See all EDG3 products (5) ...
Description
Rabbit polyclonal to EDG3 - Aminoterminal end
Specificity
This antibody is specific for EDG3 (N term).
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: QPVRGNETLREHYQYVGKLAG conjugated to KLH, corresponding to N terminal amino acids 10/30 of Human EDG3
QPVRGNETLR EHYQYVGKLA G
Positive control
Mouse kidney lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Hypertension >> Vasoconstriction >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
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Western blot - EDG3 antibody (ab38324)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Target
Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Tissue specificity
Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ99500
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EDG 3 antibody
- EDG3 antibody
- Endothelial differentiation G protein coupled receptor 3 antibody
see all
Database links
- Entrez Gene: 1903 Human
- Entrez Gene: 13610 Mouse
- Omim: 601965 Human
- SwissProt: Q99500 Human
- SwissProt: Q9Z0U9 Mouse
- Unigene: 585118 Human
- Unigene: 136736 Mouse
Anti-EDG3 antibody - Aminoterminal end images:
Western blot - EDG3 antibody (ab38324)
Anti-EDG3 antibody - Aminoterminal end (ab38324) at 1/100 dilution + mouse kidney lysate
Predicted band size : 42 kDa
Observed band size : 42 kDa
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
References for Anti-EDG3 antibody - Aminoterminal end (ab38324)
This product has been referenced in:
- Benamer Net al. Electrophysiological and functional effects of sphingosine-1-phosphate in mouse ventricular fibroblasts. Biochem Biophys Res Commun 408:6-11 (2011). WB; Mouse.Read more (PubMed: 21420933) »
- Illuzzi Get al. Sphingosine kinase mediates resistance to the synthetic retinoid N-(4-hydroxyphenyl)retinamide in human ovarian cancer cells. J Biol Chem 285:18594-602 (2010). WB; Human.Read more (PubMed: 20404323) »
See all 3 publications for this product
Publishing research using ab38324? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"