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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Overview
Product name
Anti-EDG3 antibody
See all EDG3 products (5) ...
Description
Rabbit polyclonal to EDG3
Tested applications
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human EDG3.
(Peptide available as ab74476.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa, U-87 MG, SW480 and Caco 2
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Hypertension >> Vasoconstriction >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
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Western blot - Anti-EDG3 antibody (ab74477)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 52 kDa (predicted molecular weight: 42 kDa).
Target
Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Tissue specificity
Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ99500
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EDG 3 antibody
- EDG3 antibody
- Endothelial differentiation G protein coupled receptor 3 antibody
see all
Database links
- Entrez Gene: 1903 Human
- Omim: 601965 Human
- SwissProt: Q99500 Human
- Unigene: 585118 Human
Anti-EDG3 antibody images:
Western blot - Anti-EDG3 antibody (ab74477)
All lanes : Anti-EDG3 antibody (ab74477) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
Additional bands at : 52 kDa (possible glycosylated form).
Exposure time : 20 minutes
EDG3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References for Anti-EDG3 antibody (ab74477)
ab74477 has not yet been referenced specifically in any publications.
Publishing research using ab74477? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"