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Product Name
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EDG3 antibody
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See all EDG3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EDG3
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human EDG3. (Peptide available as ab74476.)
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following whole cell lysates:
HeLa, U-87 MG, SW480 and Caco 2
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Cellular localization
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Integral Membrane Protein
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Research areas
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Cardiovascular >> Atherosclerosis >> Hypertension >> Vasoconstriction >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
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Relevance
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EDG3 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG3 is sphingosine-1-phosphate. There are eight known members of the EDG receptor family and they are implicated in mediating growth-related effects such as induction of cellular proliferation, alterations in differentiation and survival, and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C, and several protein tyrosine kinases. EDG3 is expressed in heart, placenta, kidney, and liver tissue.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.700 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EDG3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EDG3 antibody images, references, products related to ab74477 and other tools.
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EDG3 antibody images:
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 Western blot - (ab74477)
All lanes : EDG3 antibody (ab74477) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
Additional bands at : 52 kDa (possible glycosylated form).
Exposure time : 20 minutes
EDG3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Search PubMed (MEDLINE) for references to EDG3
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EDG3 antibody - more information
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Customer reviews (feedback) regarding EDG3 antibody |
Customer FAQs regarding EDG3 antibody |
Protocols for EDG3 antibody |
Price and availability of products related to EDG3 antibody |
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EDG3 antibody - related products:
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EDG3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about EDG3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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