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Products:Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Overview
Product name
Anti-EDG4 antibody - Aminoterminal end
See all EDG4 products (2) ...
Description
Rabbit polyclonal to EDG4 - Aminoterminal end
Specificity
This antibody is specific for EDG4 (N term).
Tested applications
ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide: NETIGFFYNNSGKELSSHWRP conjugated to KLH, corresponding to N terminal amino acids 10/30 of Human EDG4
NETIGFFYNN SGKELSSHWR P
Positive control
Breast carcinoma tissue and HL60 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> LIM
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500.Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Target
Relevance
EDG4 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. There are eight known members of the EDG receptor family and they are implicated in mediating growth-related effects such as induction of cellular proliferation, alterations in differentiation and survival, and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C, and several protein tyrosine kinases. EDG4 is expressed in testes, ovarian tumor, and leukocyte containing tissues.
Cellular localization
Plasma membrane
Alternative names
- Endothelial Cell Differentiation Gene 4 antibody
- Endothelial Cell Differentiation Gene 4 antibody
- Endothelial differentiation lysophosphatidic acid G protein coupled receptor 4 antibody
see all
Database links
- Entrez Gene: 9170 Human
- Entrez Gene: 53978 Mouse
- Omim: 605110 Human
- SwissProt: Q9HBW0 Human
- SwissProt: Q9JL06 Mouse
- Unigene: 122575 Human
- Unigene: 23253 Mouse
Anti-EDG4 antibody - Aminoterminal end images:
Western blot - EDG4 antibody (ab38322)
Anti-EDG4 antibody - Aminoterminal end (ab38322) at 1/100 dilution + HL60 cell lysate
Predicted band size : 39 kDa
Observed band size : 39 kDa
Additional bands at : 15 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EDG4 antibody (ab38322)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38322 at a 1/50 dilution.
References for Anti-EDG4 antibody - Aminoterminal end (ab38322)
ab38322 has not yet been referenced specifically in any publications.
Publishing research using ab38322? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"