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Anti-EDR1 / PHC1 antibody (ab52674)

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Overview

Product name

Anti-EDR1 / PHC1 antibody
See all EDR1 / PHC1 products (3) ...

Description

Mouse polyclonal to EDR1 / PHC1

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse

Immunogen

Recombinant fragment (Mouse)

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol, Whole serum

Purity

Whole antiserum

Primary antibody notes

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - PHC 1 / EDR 1 antibody (ab52674)Western blot - PHC 1 / EDR 1 antibody (ab52674) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: 1/1000. Predicted molecular weight: 105 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

PHC1 is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The Polycomb group (PcG) genes are essential for maintenance of appropriate expression patterns of developmental master regulators, such as Hox genes, and thus are essential for proper development. PHC1 is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. PHC1, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein.

Cellular localization

Nuclear

Alternative names

  • Early development regulatory protein 1 antibody
  • hPH1 antibody
  • EDR1 PH1 antibody
  • mPH1 antibody
  • PH1 antibody
  • Polyhomeotic-like protein 1 antibody
  • Rae28 antibody
see all

Database links

Anti-EDR1 / PHC1 antibody images:

  Western blot - PHC 1 / EDR 1 antibody (ab52674)

Western blot - PHC 1 / EDR 1 antibody (ab52674)

All lanes : Anti-EDR1 / PHC1 antibody (ab52674) at 1/1000 dilution

Lane 1 : protein extract from E coli with ~50ng to 500ng of antigen (tagged-antigen fusion protein).
Lane 2 : protein extract from E coli with ~50ng to 500ng of of an irrelevant antigen

Lysates/proteins at 20 µg per lane.

Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase at 1/5000 dilution

Predicted band size : 105 kDa

References for Anti-EDR1 / PHC1 antibody (ab52674)

ab52674 has not yet been referenced specifically in any publications.

Publishing research using ab52674? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"