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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC1
Anti-EDR1 / PHC1 antibody
See all EDR1 / PHC1 products (3) ...
Mouse polyclonal to EDR1 / PHC1
Reacts with
Mouse
Recombinant fragment (Mouse)
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 50% Glycerol, Whole serum
Whole antiserum
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Polyclonal
IgG
Western blot - PHC 1 / EDR 1 antibody (ab52674)
(enlarge)
Our Abpromise guarantee covers the use of ab52674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 105 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
PHC1 is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The Polycomb group (PcG) genes are essential for maintenance of appropriate expression patterns of developmental master regulators, such as Hox genes, and thus are essential for proper development. PHC1 is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. PHC1, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein.
Nuclear
Western blot - PHC 1 / EDR 1 antibody (ab52674)

All lanes : Anti-EDR1 / PHC1 antibody (ab52674) at 1/1000 dilution
Lane 1 : protein extract from E coli with ~50ng to 500ng of antigen (tagged-antigen fusion protein).
Lane 2 : protein extract from E coli with ~50ng to 500ng of of an irrelevant antigen
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase at 1/5000 dilution
Predicted band size : 105 kDa
ab52674 has not yet been referenced specifically in any publications.
Publishing research using ab52674? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-EDR1 / PHC1 antibody (ab52674) at 1/1000 dilution
Lane 1 : protein extract from E coli with ~50ng to 500ng of antigen (tagged-antigen fusion protein).
Lane 2 : protein extract from E coli with ~50ng to 500ng of of an irrelevant antigen
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase at 1/5000 dilution
Predicted band size : 105 kDa
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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