![Western blot - EEA1 antibody [EPR4245] (ab109110) image](#EEA1-Primary-antibodies-ab109110-2.jpg)
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Product name
Anti-EEA1 antibody [EPR4245]
See all EEA1 products (9) ...
Description
Rabbit monoclonal [EPR4245] to EEA1
Tested applications
WB, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues in the N terminus of Human EEA1 (UniProt Q15075).
Positive control
COS-1, NIH 3T3, C6, HeLa, Jurkat, and JAR cell lysates
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR4245
Isotype
IgG1
Research areas
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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Western blot - EEA1 antibody [EPR4245] (ab109110)
![Western blot - EEA1 antibody [EPR4245] (ab109110) image](/ps/datasheet/images/109/ab109110/EEA1-Primary-antibodies-ab109110-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab109110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/10000 - 1/50000. Detects a band of approximately 170 kDa (predicted molecular weight: 162 kDa).
ICC
ICC: 1/500 - 1/1000.ICC: 1/500 - 1/1000.
Application notes
Is unsuitable for ,Flow Cyt,IHC-P or IP.
Target
Function
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 FYVE-type zinc finger.
Domain
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
Cellular localization
Cytoplasm. Early endosome membrane.
Target information above from: UniProt accessionQ15075
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Early endosome antigen 1 antibody
- Early endosome antigen 1, 162kD antibody
- Early endosome associated protein antibody
see all
Database links
- Entrez Gene: 8411 Human
- Entrez Gene: 216238 Mouse
- Entrez Gene: 314764 Rat
- Omim: 605070 Human
- SwissProt: Q15075 Human
- SwissProt: Q8BL66 Mouse
- Unigene: 567367 Human
- Unigene: 210035 Mouse
see all
Anti-EEA1 antibody [EPR4245] images:
Western blot - EEA1 antibody [EPR4245] (ab109110)
All lanes : Anti-EEA1 antibody [EPR4245] (ab109110) at 1/10000 dilution
Lane 1 : COS-1 cell lysate
Lane 2 : NIH 3T3 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 162 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
References for Anti-EEA1 antibody [EPR4245] (ab109110)
ab109110 has not yet been referenced specifically in any publications.
Publishing research using ab109110? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"