EEA1 antibody (ab28503)

Product Name 

EEA1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to EEA1

Immunogen 

Synthetic peptide derived from residues 307 - 356 of human EEA1.

.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 0.06 µg/ml. Predicted molecular weight: 162 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Placenta tissue lysate.

Cellular localization 

Endosome

Research areas 

Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker

Relevance 

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, similar to those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown to be dependent on phosphatidyl 3 kinase activity; inhibitors of this enzyme cause EEA1 to dissociate from early endosomes.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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