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My technician is currently doing additional testing with ab50313. Because I will be out of the country until Nov 17, I would prefer to get back to you after my return in conjunction with those new results. I hope this delay is OK, but please let me know of there is a problem with this. |
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ANSWER: |
Thanks for the update. We certainly appreciate your lab's troubleshooting efforts. Please feel free to reply after your trip. |
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High background staining human and rat cells. |
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ANSWER: |
We have previously been in contact regarding ab2074, which was not providing satisfactory results.
We had offered a free of charge replacement with a different antibody, but we were waiting to hear from you with your selection. If you have determined which antibody you would like as a replacement, please let us know and we will send it promptly.
I wish you the best of luck with your research and please do not hesitate to contact us if you have any further questions. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
HeLa cells fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 µg/mL ab50313."
ab50313 staining EEA1 in mouse primary astrocytes by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocking with 10% serum was performed for 20 minutes at 250C. Samples were incubated with primary antibody (1/150: in 10% goat serum / PBS) for 1 hour at 25°C. An Abcam`s ab6939, Cy3®-conjugated goat polyclonal to rabbit IgG (H&L) was used as secondary antibody at 1/200 dilution.
This image is a courtesy of Anonymous Abreview
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