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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Overview
Product name
Anti-EEA1 antibody
See all EEA1 products (9) ...
Description
Rabbit polyclonal to EEA1
Tested applications
WB, ICC, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: GSQGSDLDSSATPINTVDVNNESSSEG conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 24 - 40 of Human EEA1
GSQGSDLDSS ATPINTVDVN NESSSEG
Positive control
whole extract of mouse NIH-3T3 cells; human HeLa and rat NRK cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ICC/IF
Three stars (1 Abreview)
Application notes
ICC/IF: Use at a concentration of 5 - 10 µg/ml.
WB: Use at a concentration of 0.4 - 0.8 µg/ml. Detects a band of approximately 160 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 FYVE-type zinc finger.
Domain
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
Cellular localization
Cytoplasm. Early endosome membrane.
Target information above from: UniProt accessionQ15075
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Early endosome antigen 1 antibody
- Early endosome antigen 1, 162kD antibody
- Early endosome associated protein antibody
see all
Database links
- Entrez Gene: 8411 Human
- Entrez Gene: 216238 Mouse
- Entrez Gene: 314764 Rat
- Omim: 605070 Human
- SwissProt: Q15075 Human
- SwissProt: Q8BL66 Mouse
- Unigene: 567367 Human
- Unigene: 210035 Mouse
see all
Anti-EEA1 antibody images:
Immunofluorescence - EEA1 antibody (ab50313)
HeLa cells fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 µg/mL ab50313."
Immunocytochemistry - EEA1 antibody (ab50313)
ab50313 staining EEA1 in mouse primary astrocytes by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocking with 10% serum was performed for 20 minutes at 250C. Samples were incubated with primary antibody (1/150: in 10% goat serum / PBS) for 1 hour at 25°C. An Abcam`s ab6939, Cy3®-conjugated goat polyclonal to rabbit IgG (H&L) was used as secondary antibody at 1/200 dilution.
This image is a courtesy of Anonymous Abreview
References for Anti-EEA1 antibody (ab50313)
This product has been referenced in:
- Torres VAet al. Rab5 mediates caspase-8-promoted cell motility and metastasis. Mol Biol Cell 21:369-76 (2010). WB; Human.Read more (PubMed: 19923319) »
- Ahtiainen Let al. Defects in innate immunity render breast cancer initiating cells permissive to oncolytic adenovirus. PLoS One 5:e13859 (2010). ICC/IF; Human.Read more (PubMed: 21079774) »
See all 3 publications for this product
Publishing research using ab50313? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"