Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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ab18175 has been referenced in 1 publications.
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All lanes : Anti-EEA1 antibody [mAbcam18175] (ab18175) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 :
Lane 3 :
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 162 kDa
Overlay histogram showing HeLa cells stained with ab18175 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18175, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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