EEA1 antibody [mAbcam18175] (ab18175)

Product Name 

EEA1 antibody [mAbcam18175]

Product type 

Primary antibodies

Description 

Mouse monoclonal [mAbcam18175] to EEA1

Immunogen 

Synthetic peptide derived from within residues 1350 to the C-terminus of Human EEA1.

(Peptide available as ab14946.)

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat, Chk, Chmp, Cow, Dog
(Due to sequence homology)

Specificity 

Detects a band at 160 kDa that represents EEA1 in Western blotting on human cell lines. Also detects a band at 100 kDa, we are unsure as to the identity of this band.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 162 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Endosome

Research areas 

Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker

Relevance 

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, similar to those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown to be dependent on phosphatidyl 3 kinase activity; inhibitors of this enzyme cause EEA1 to dissociate from early endosomes.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

mAbcam18175

Isotype 

IgG2b

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.5

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

2.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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