You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-EEA1 antibody [mAbcam18175] (ab18175)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-EEA1 antibody [mAbcam18175]
See all EEA1 products (9) ...

Description

Mouse monoclonal [mAbcam18175] to EEA1

Specificity

Detects a band at 160 kDa that represents EEA1 in Western blotting on human cell lines. Also detects a band at 100 kDa, we are unsure as to the identity of this band.

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat, Chicken, Cow, Dog, Chimpanzee, Zebrafish

Immunogen

Synthetic peptide derived from within residues 1350 to the C-terminus of Human EEA1.

Read Abcam's proprietary immunogen policy

(Peptide available as ab14946.)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.5

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

mAbcam18175

Isotype

IgG2b

Light chain type

kappa

Research areas

Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker

  • Western blot - EEA1 antibody [mAbcam18175] (ab18175)Western blot - EEA1 antibody [mAbcam18175] (ab18175) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab18175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Sequence similarities

Contains 1 C2H2-type zinc finger.
Contains 1 FYVE-type zinc finger.

Domain

The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.

Cellular localization

Cytoplasm. Early endosome membrane.

Target information above from: UniProt accessionQ15075 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Early endosome antigen 1 antibody
  • Early endosome antigen 1, 162kD antibody
  • Early endosome associated protein antibody
  • Early endosome associated protein antibody
  • EEA 1 antibody
  • EEA1 antibody
  • EEA1_HUMAN antibody
  • Endosome associated protein p162 antibody
  • Endosome-associated protein p162 antibody
  • MST105 antibody
  • MSTP105 antibody
  • ZFYVE2 antibody
  • ZFYVE2 antibody
  • Zinc finger FYVE domain containing protein 2 antibody
  • Zinc finger FYVE domain-containing protein 2 antibody
see all

Database links

see all

Anti-EEA1 antibody [mAbcam18175] images:

  Western blot - EEA1 antibody [mAbcam18175] (ab18175)

Western blot - EEA1 antibody [mAbcam18175] (ab18175)

All lanes : Anti-EEA1 antibody [mAbcam18175] (ab18175) at 1 µg/ml

Lane 1 : HeLa whole cell lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)

Lysates/proteins at 20 µg per lane.

Secondary
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 162 kDa

See Abreview

References for Anti-EEA1 antibody [mAbcam18175] (ab18175)

This product has been referenced in:

  • Zhou Het al. Analysis of the subcellular phosphoproteome using a novel phosphoproteomic reactor. J Proteome Res 9:1279-88 (2010).Read more (PubMed: 20067319) »

See 1 publication for this product

Publishing research using ab18175? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"