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Western blot - EED antibody (ab14294)
(enlarge)
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Product Name
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EED antibody
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See all EED antibodies (2)...
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to EED
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Immunogen
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Synthetic peptide: AVSIESGTNT ERPDTPTNTP NAPGRKSWGK GKWSSKKCKY SFKCVNSLKE DHNQPLFGVQ FNWHSKEGDP LVFATVGSNRVTLYECHSQG EIRLLQSYVD ADADENFYTC AWTYDSNTSH PLLAVAGS, corresponding to amino acids 41-168 of Human EED.
BLAST 'AVSIESGTNT ERPDTPTNT P NAPGRKSW GK GKWSSKK CKY SFKCVN SLKE DHNQP LFGVQ FNWH SKEGDP LVF ATVGSNRVTL YECHSQG EI RLLQSYVD A DADENFYTC AWTYDSNTSH ' with  or 
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Reacts with
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Hu, Ms, Rat
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000 - 1/2000. Predicted molecular weight: 53 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC3
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
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Relevance
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This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Four transcript variants encoding distinct isoforms have been identified for this gene. The four isoforms are differentialy expressed during ES cell differentiation, with four isoforms intially expressed. Only isoform 3 then remains after differentiation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EED antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EED antibody images, references, products related to ab14294 and other tools.
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EED antibody images:
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 Western blot - EED antibody (ab14294)
Predicted band size : 53 kDa
E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. Arrow points to EED fusion protein.
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References for EED antibody (ab14294)
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This product has been used in:
Trojer P et al. Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins. J Biol Chem 284:8395-405 (2009). PubMed: 19144645
If you publish research using ab14294 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to EED
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EED antibody - more information
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Customer reviews (feedback) regarding EED antibody |
Customer FAQs regarding EED antibody |
Protocols for EED antibody |
Price and availability of products related to EED antibody |
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EED antibody - related products:
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EED antibody - other tools:
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Contact Abcam with a Technical Enquiry about EED antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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