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ab13743 |
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ab13743 |
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Read our guarantee »Publishing research using ab4469? Please let us know so that we can cite the reference in this datasheet
ab4469 has been referenced in 6 publications.
Publishing research using ab4469? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Rabbit polyclonal to EED (ab4469) on U20S cells. Antibody used at 1/100.
U20S cells were fixed with PFA (3.7%), permeabilised with PBS 0.1% Triton. Counterstained with Hoechst. Cells were examined with Axioplan 2 microscope (Zeiss).
Secondary antibody: donkey anti-rabbit (Cy3).
Predicted band size : 49 kDa
Rabbit polyclonal to EED (ab4469) at 1/200.
Lane 1: WB on HeLa nuclear extract (250ug).
Lanes 2-5: IP - U20S cells were transfected with 4ug of HA-EED. WB using TBS milk 2%, BSA 0.5%.
Anti-EED antibody (ab4469) at 1/250 dilution + EED Recombinant Protein (Human) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
ab4469 recognizes the full-length recombinant EED protein which has an expected molecular weight of 80 kDa (tagged).
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