|
ab13743 |
|
|
ab13743 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab4469 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
I had tried 5 times used the ab4469 antibody and It did not work. And I had tried 5 times, so I bought another antibody UPSTATE:17-663, and had got the anticipate result. The condition were as follows: The concentration of the primary antibody was 1:1000; The concentration of the secondary antibody was 1:5000;
So I thought there was something wrong with this antibody and did not want to try again.
|
|||||||||
ANSWER: |
Thank you for contacting us.
I am sorry that the results did not improve. I would like to ask how you would like to proceed with this case. In order to help you further I would need more information about order number. The order number you have provided does not match actual Abcam order. It looks like you are from China and you may have purchased this antibody from our Chinese distributor. Please contact them for contacting us for further instructions.
Please be advised that I can only provide free of charge replacement or refund/ credit note if the antibody was ordered within Abcam guarantee period; which is 4 month for Chinese customers.
If you are not from China please accept my apologies for misunderstanding and provide me the correct 6 digit Abcam order number.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Rabbit polyclonal to EED (ab4469) on U20S cells. Antibody used at 1/100.
U20S cells were fixed with PFA (3.7%), permeabilised with PBS 0.1% Triton. Counterstained with Hoechst. Cells were examined with Axioplan 2 microscope (Zeiss).
Secondary antibody: donkey anti-rabbit (Cy3).
Predicted band size : 49 kDa
Rabbit polyclonal to EED (ab4469) at 1/200.
Lane 1: WB on HeLa nuclear extract (250ug).
Lanes 2-5: IP - U20S cells were transfected with 4ug of HA-EED. WB using TBS milk 2%, BSA 0.5%.
Anti-EED antibody (ab4469) at 1/250 dilution + EED Recombinant Protein (Human) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
ab4469 recognizes the full-length recombinant EED protein which has an expected molecular weight of 80 kDa (tagged).
0
Call 01223 696 000 or contact us