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Overview
Product name
Anti-EED antibody
See all EED products (3) ...
Description
Rabbit polyclonal to EED
Specificity
ab4469 specifically recognises the four isoforms of EED following the immunoprecipitation of tagged Ezh2 from HEK293 nuclear extract. However, using HEK293 nuclear lysate ab4469 predominantly recognises non-specific bands.
Tested applications
WB, IP, ICC/IF, IHC-FoFrmore details
Cross reactivity
Reacts with
Cow, Human
Predicted to work with
Mouse, Chicken, Xenopus laevis
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human EED.
(Peptide available as ab13743.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC3
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/100.
IHC-FoFr: Use at an assay dependent dilution (PMID 18784248).
IP: Use at an assay dependent dilution.
WB: 1/200. Predicted molecular weight: 49 kDa. Can be blocked with EED peptide (ab13743).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Tissue specificity
Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
Sequence similarities
Belongs to the WD repeat ESC family.
Contains 7 WD repeats.
Developmental stage
Expression peaks at the G1/S phase boundary.
Cellular localization
Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
Target information above from: UniProt accessionO75530
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- eed antibody
- EED protein antibody
- EED_HUMAN antibody
see all
Database links
- Entrez Gene: 426381 Chicken
- Entrez Gene: 404183 Cow
- Entrez Gene: 8726 Human
- Entrez Gene: 13626 Mouse
- Entrez Gene: 398420 Xenopus laevis
- Omim: 605984 Human
- SwissProt: Q5ZKH3 Chicken
- SwissProt: Q3SZ25 Cow
see all
Anti-EED antibody images:
Immunocytochemistry/ Immunofluorescence - EED antibody (ab4469)
Rabbit polyclonal to EED (ab4469) on U20S cells. Antibody used at 1/100.
U20S cells were fixed with PFA (3.7%), permeabilised with PBS 0.1% Triton. Counterstained with Hoechst. Cells were examined with Axioplan 2 microscope (Zeiss).
Secondary antibody: donkey anti-rabbit (Cy3).
Western blot - EED antibody (ab4469)
Predicted band size : 49 kDa
Rabbit polyclonal to EED (ab4469) at 1/200.
Lane 1: WB on HeLa nuclear extract (250ug).
Lanes 2-5: IP - U20S cells were transfected with 4ug of HA-EED. WB using TBS milk 2%, BSA 0.5%.
Western blot - EED antibody (ab4469)
Anti-EED antibody (ab4469) at 1/250 dilution + EED Recombinant Protein (Human) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
ab4469 recognizes the full-length recombinant EED protein which has an expected molecular weight of 80 kDa (tagged).
References for Anti-EED antibody (ab4469)
This product has been referenced in:
- Yap DBet al. Somatic mutations at EZH2 Y641 act dominantly through a mechanism of selectively altered PRC2 catalytic activity, to increase H3K27 trimethylation. Blood 117:2451-9 (2011). WB; Human.Read more (PubMed: 21190999) »
- Borbone Eet al. Enhancer of zeste homolog 2 overexpression has a role in the development of anaplastic thyroid carcinomas. J Clin Endocrinol Metab 96:1029-38 (2011).Read more (PubMed: 21289264) »
See all 6 publications for this product
Publishing research using ab4469? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"