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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - EEF1A2 antibody (ab82912)
Anti-EEF1A2 antibody (ab82912) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
Gel concentration 12%
Western blot - Anti-EEF1A2 antibody (ab82912)
All lanes : Anti-EEF1A2 antibody (ab82912) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
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